Figure 3

Molecular effects of resveratrol on anti-apoptotic proteins. Effects of resveratrol on (A) Sirt1 and (B) Ku70 expression in antibody-treated E1A-NR3 cells. The cells were pretreated with 40 μM resveratrol for 16 hrs followed by 2 hrs incubation in Enol-1 or Rec-1 antibodies. The protein cell extracts were analyzed by Western blotting using anti-Sirt1 and anti-Ku70 antibodies. Expression level is presented as a fold increase calculated from the intensity of each band compared to the untreated controls adjusted for the loading control using a densitometry analyses. The bars represent an average of three experiments.