Table 4 PCR primers, forward (F) and reverse (R) listed 5' to 3', PCR product size in base pairs, and base pair position within the PCR product for the 13 SNPs concordant with the polled trait in Holsteins.
From: Single nucleotide polymorphisms concordant with the horned/polled trait in Holsteins
SNP Name | Primer Set | PCR Size | Position |
|---|---|---|---|
bC2163_AG_454 | F: CTTGCCCTTGACTTTCTCTC | 501 | 454 |
R: CTCTCCTCCTCCTCAGTTTG | |||
bC2163_GA_333 | F: GATTCACCACCACACTGGA | 740 | 333 |
R: CACCATCACAAAGCAGAAAA | |||
bC2163_GA_252 | F: GGCAATGTCACCATCAACC | 383 | 252 |
R: CAAAGAACAGAAAGCCAACAAG | |||
bC2163_AC_126 | F: TCTAAGTGCCTGTAATCTGTGAG | 563 | 126 |
R: AGGTCTTTTGCGGTGTAATC | |||
bC2163_CT_145 | F: TCTAAGTGCCTGTAATCTGTGAG | 563 | 145 |
R: AGGTCTTTTGCGGTGTAATC | |||
bCLCVS1_TC_238 | F: CTAAGGAATTTGACTCTCACCTCT | 567 | 238 |
R: CTACTCCCCTTCTGCTACCC | |||
bCLF1O1_GA_193 | F: CTGGTCGGTTTGAGGGTTG | 559 | 193 |
R: AGCAGGAACTGGCTCTCGT | |||
bC2159_C-193T | F: CTGGTCGGTTTGAGGGTTG | 559 | 320 |
R: AGCAGGAACTGGCTCTCGT | |||
bC2159_T372C | F: TACTTTCTCTCATCTCACTTCCTC | 370 | 165 |
R: GTTTGTTTCCTTTGCCTCTG | |||
bSYNJ1_CT_271 | F: TCACTGGATGTATGTCTGTTGG | 461 | 271 |
R: TCAGGAAGAGTAAATGGGTTTC | |||
bSYNJ1_CT_131 | F: TCAGTATTTGTGGCATGTGG | 508 | 131 |
R: CTCGACTTGGTCTCACTGG | |||
bSYNJ1_AG_244 | F: GAATGACAAAGAGGCAGAGG | 497 | 244 |
R: AGAGCTGGCCCTAAAGAAAG | |||
bSYNJ1_C3981T | F: AACCACCAGAGTAACAGACTACAC | 471 | 190 |
R: CTGTCGGTGAAAGGATTTG |