Figure 5

RT-PCR analyses of selected genes ( Zfp711, Lox, Npal1, Spata6, Ptrf, Tgfbr1, F13b, Vstm2, Slc2a2 and Tnfaip8 ) of Pkd1^{L 3/L 3}and control mice at different stages. RNA was extracted from Pkd1^{L 3/L 3}mice and age-matched control littermates at PNW 1, 2, 3 and 3.5. Three micrograms of total RNA from each group were reverse transcribed and cDNA equivalent to 10 ng total RNA was used for the PCR reactions. The graphs represent the relative change compared to control groups after normalization against Gapdh expression (mean ± SD of triplicate PCR reactions of pooled RNA; n = 3; *, P < 0.05; **, P < 0.01 compared with wild-type mice, by Student's t-test).