Synergistic effect of methyljasmonate and cyclodextrin on stilbene biosynthesis pathway gene expression and resveratrol production in Monastrell grapevine cell cultures
- Diego Lijavetzky†1, 2,
- Lorena Almagro†3,
- Sarai Belchi-Navarro3,
- José M Martínez-Zapater1, 4,
- Roque Bru5 and
- Maria A Pedreño3Email author
© Pedreño et al; licensee BioMed Central Ltd. 2008
Received: 11 July 2008
Accepted: 22 December 2008
Published: 22 December 2008
Plant cell cultures have been shown as feasible systems for the production of secondary metabolites, being the elicitation with biotic or abiotic stimuli the most efficient strategy to increase the production of those metabolites. Vitaceae phytoalexins constitute a group of molecules belonging to the stilbene family which are derivatives of the trans-resveratrol structure and are produced by plants and cell cultures as a response to biotic and abiotic stresses. The potential benefits of resveratrol on human health have made it one of the most thoroughly studied phytochemical molecules. The aim of this study was to evaluate the elicitor effect of both cyclodextrin (CD) and methyljasmonate (MeJA) on grapevine cell cultures by carrying out a quantitative analysis of their role on resveratrol production and on the expression of stilbene biosynthetic genes in Vitis vinifera cv Monastrell albino cell suspension cultures.
MeJA and CD significantly but transiently induced the expression of stilbene biosynthetic genes when independently used to treat grapevine cells. This expression correlated with resveratrol production in CD-treated cells but not in MeJA-treated cells, which growth was drastically affected. In the combined treatment of CD and MeJA cell growth was similarly affected, however resveratrol production was almost one order of magnitude higher, in correlation with maximum expression values for stilbene biosynthetic genes.
The effect of MeJA on cell division combined with a true and strong elicitor like CD could be responsible for the observed synergistic effect of both compounds on resveratrol production and on the expression of genes in the stilbene pathway.
The more relevant Vitaceae phytoalexins comprise a group of molecules belonging to the stilbene family [1, 2], which are derivatives of the trans-resveratrol structure (3,5,4'-trihydroxystilbene). In addition to trans-resveratrol derived molecules, other oligomers produced by its oxidation and generically known as viniferins have been found as the result of infection or stress . Different naturally occurring stilbenes like resveratrol, pterostilbene, piceatannol and resveratrol glucoside derivatives  are known to be strong antioxidants. In particular, the potential benefits of resveratrol on human health have made it one of the most thoroughly studied phytochemical molecules . See de la Lastra and Villegas  for a review of the reported resveratrol effects.
Stilbenes are synthesized via the phenylpropanoid/malonate pathway from phenylalanine that, in turn, is converted into cinnamic acid by phenylalanine ammonia lyase (PAL). The consecutive action of cinnamate 4-hydroxylase (C4H) and 4-coumarate CoA ligase (4CL) transform cinnamic acid into p-coumaryl-CoA. Derived compounds, collectively referred to as polyphenols, are originated at this branching point through the action of enzymes chalcone synthase (CHS) and stilbene synthase (STS) for flavonoids and stilbenoids, respectively .
Vitis vinifera cell cultures have been used in several studies to explore the factors involved in the induction and regulation of stilbene biosynthesis and metabolism [7, 8]. Jasmonic acid (JA) and its more active derivative methyljasmonate (MeJA) have been proposed as key compounds of the signal transduction pathway involved in the elicitation of secondary metabolite biosynthesis which takes part in plant defence reactions . Application of MeJA on grapevine leaves and plant cell suspension cultures can induce the accumulation of stilbenes [10, 11]. However, the reported amount of stilbenes secreted to the medium in MeJA-treated cell cultures is negligible [8, 12].
Cyclodextrins (CDs) are naturally occurring cyclic oligosaccharides derived from starch. Addition of 2,6 dimethyl-β-ciclodextrin (DIMEB) to grapevine cell cultures induces both resveratrol biosynthesis and its accumulation in the culture media . Among the differently modified β-cyclodextrins, the methylated and hydroxypropylated caused the highest production of this phytoalexin, which is translocated to the cell walls and accumulates in the culture media [14, 15].
The aim of this study was to evaluate the elicitor effect of joint applications of CDs and MeJA on grapevine cell cultures by carrying out a quantitative analysis of their role on resveratrol production. We also monitored the expression of several genes encoding key enzymes in the phenylpropanoid pathway, including those involved in resveratrol biosynthesis, to determine the relationship between resveratrol accumulation in the medium after elicitation and the regulation of gene expression. A synergistic interaction between CD and MeJA on resveratrol production and on the expression of stilbene biosynthesis related genes is discussed.
MeJA but not CD affects cell growth
CD and MeJA synergistically induce resveratrol production
CD and MeJA synergistically and specifically induce the expression of STS and the general phenylpropanoid pathway
To evaluate the specificity of the stilbene pathway induction, we analyzed the relative expression of the general phenylpropanoid pathway genes (PAL, C4H, 4CL) as well as that of CCR and CHS in treated cell cultures. These last two genes encode key enzymes of stilbene alternative pathways: CHS catalyzes the first step in the anthocyanin and isoflavonoid biosynthetic pathways, and CCR plays a similar role in the lignin biosynthetic pathway. Gene expression was evaluated at the same time points described above. The expression profiles of PAL, C4H, and 4CL were almost identical to those of STS with an earlier induction in the case of the combined treatment of CD and MeJA (Figure 3). On the other hand, the relative expression of CCR was very low, about one order of magnitude lower than that of the less expressed gene analyzed in the pathway (i.e. 4CL, Figure 3). Moreover, none of two CHS assayed genes displayed any detectable expression along the whole experiment (Figure 3).
The results described here show that the combined addition of MeJA and CD to V. vinifera cv. Monastrell albino cell cultures yields a much higher resveratrol accumulation than the sum of the individual additions. In our experiments, the combined treatment increased seven times the yield of resveratrol when compared to CDs alone. This final resveratrol level (1600 μmole gDW-1) represents an increase between 10- and more than 1000-fold with respect to previous reports [8, 12, 16]. The expression analysis of this response shows that both elicitors stimulated the expression of PAL, C4H, 4CL and STS independently of the anthocyanins/isoflavonoids (CHS) and lignins (CCR) pathways and therefore both induce stilbene biosynthetic genes in a highly specific way, in agreement to results reported by Saigne-Soulard et al.. Furthermore, the synergistic interaction of both elicitors on resveratrol production (Figure 2) seems to be the result of their synergistic effect on the expression of biosynthetic genes (Figure 3).
In MeJA-treated cells, a significant reduction in cell growth was observed (Figure 1) in parallel with a strong induction of the general phenylpropanoid pathway (Figure 3), as recently reported for Arabidopsis cell suspension cultures . However, although STS expression was highly induced (Figure 3), no significant amounts of resveratrol were detected in the spent media (Figure 2). Such discrepancy could be due to either post-transcriptional and/or post-translational regulatory mechanisms [12, 16, 19].
CD elicited cells effectively produced significant amounts of resveratrol (Figure 2) in correlation with a transient expression of the central phenylpropanoid enzymes and STS genes (Figure 3). Furthermore, most (or all) the resveratrol synthesized up to 72 h remained in the culture medium until the end of the assay (Figure 2). CDs are able to form inclusion complexes with stilbene compounds, such as trans- resveratrol and diethylstilbestrol [13, 20], which could protect resveratrol from oxidation or glucosylation. This could explain the observation that, although STS expression dropped after 72 h in the CD treatment (Figure 3), the amount of resveratrol stayed constant (Figure 2). In contrast to the effect of MeJA, CD treated cells were not altered in their growth, displaying a similar biomass growth curve as control cells (Figure 1). Since both complexed and uncomplexed CD molecules remain in the culture medium during the whole assay, the transient gene expression and the high but limited production of resveratrol must be the result of additional regulatory mechanisms. Given the regular growth curve of the CD-treated cell cultures it is tentative to propose that engagement of cells in active division could somehow compete with further production of resveratrol after a transient elicitation response. In fact, Naill & Roberts  observed that most metabolite productive cells in Taxus cuspidata suspension cultures were in the G0/G1 phase of the cell cycle (i.e. non-cycling cells) and this stage was suggested as the most specialised for accumulation of secondary metabolites.
In agreement with the previous hypothesis, when both CD and MeJA were simultaneously added to the culture medium, they caused a significant reduction in cell growth (Figure 1) as well as a sustainable maximum expression of STS and central phenylpropanoid genes, even after 168 h (Figure 3), which was paralleled by a maximum resveratrol accumulation in the medium (Figure 2). We believe that the blockage in cell division and metabolic rearrangement likely caused by MeJA  could place the cells in a non-cycling state  allowing a sustained elicitation by CD.
It has been suggested that MeJA may induce a subset of secondary metabolite biosynthetic genes which could modulate expression of genes and accumulation of compounds induced by elicitors [22, 23]. Although we cannot completely discard this possibility, we show that the synergistic effect observed on resveratrol production is related with a synergistic effect on the expression of the same set of stilbene biosynthetic genes induced by CD (Figure 3). The observed effects of MeJA on cell suspension growth and the recent characterization of MeJA effects on Arabidopsis cell cultures open the possibility to propose an alternative hypothesis to explain this synergy based on the combined effect of MeJA on cell cycle together with a true and strong elicitor like CD. Further experiments will be required to confirm this possibility on the interaction between cell cycle and secondary metabolite biosynthetic gene expression.
Establishment of calli and cell suspension cultures
Elicitation of Monastrell albino cell cultures
Elicitation experiments were carried out on three replicates of 14 days old grapevine cell suspensions. Washed cells (20 g FW) were transferred into 250 ml flask and resuspended in 100 ml of sterile fresh medium containing either 50 mM DIMEB, 100 μM MeJA or 50 mM DIMEB + 100 μM MeJA. Control cultures contained no additional DIMEB or MeJA. In order to assess for any effect on cell growth of the ethanol used to deliver the MeJA, we carried out biomass measures in control cell suspensions treated with 0.2% v/v ethanol during the experimental set up. There were no significant differences in cell growth between ethanol-treated and non-treated control cell suspensions (data not shown). All cell suspensions were incubated for up to 168 h at 25°C in darkness in a rotary shaker (110 rpm) and a cell growth time course was performed both for treated and control cells. After elicitation, cells were filtered from the spent medium under gentle vacuum, rapidly washed with cold distilled water, weighted and frozen at -80°C until use. The spent medium was used for stilbenoids analysis.
Analysis of stilbenoids in the spent medium and in cells
Samples were analyzed by liquid chromatography according to Dalluge et al.  with modifications in an Agilent 1100 series HPLC equipped with UV-vis and ESI-MS detectors. For more details, see Additional File 2. A time course of stilbenoid production was also performed up to 168 h of cell culture.
RNA isolation, cDNA synthesis and Real-time quantitative RT-PCR (qRT-PCR)
Primer pairs used for real time quantitative RT-PCR
Phenylalanine ammonia lyase
SBN and LA hold grants from the Fundación Séneca. We thank Lucie Fernandez for helpful comments on qRT-PCR and Pepita Alemán for maintaining Monastrell albino cell cultures. This work has been partially supported by the MEC and FEDER (BIO2005-00332), Consejería de Educación, Ciencia e Investigación de la Región de Murcia (2I05SU0020 and BIO-BVA 07/01-0003) and the GrapeGen project (a joint international project funded by Genoma España and Genome Canada).
- Langcake P, Pryce RJ: A new class of phytoalexins from grapevines. Experientia. 1977, 33 (2): 151-152. 10.1007/BF02124034.View ArticlePubMedGoogle Scholar
- Jeandet P, Douillet-Breuil AC, Bessis R, Debord S, Sbaghi M, Adrian M: Phytoalexins from the Vitaceae: biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolism. Journal of Agricultural and Food Chemistry. 2002, 50: 2731-2741. 10.1021/jf011429s.View ArticlePubMedGoogle Scholar
- Pezet R, Gindro K, Viret O, Richter H: Effects of resveratrol, viniferins and pterostilbene on Plasmopara viticola zoospore mobility and disease development. Vitis. 2004, 43: 145-148.Google Scholar
- Pezet R, Gindro K, Viret O, Spring JL: Glycosylation and oxidative dimerization of resveratrol are respectively associated to sensitivity and resistance of grapevine cultivars to downy mildew. Physiological and Molecular Plant Pathology. 2004, 65: 297-303. 10.1016/j.pmpp.2005.03.002.View ArticleGoogle Scholar
- Wieder T, Prokop A, Bagci B, Essmann F, Bernicke D, Schulze-Osthoff K, Dörken B, Schmalz HG, Daniel PT, Henze G: Piceatannol, a hydroxylated analog of the chemopreventive agent resveratrol, is a potent inducer of apoptosis in the lymphoma cell line BJAB and in primary, leukemic lymphoblasts. Leukemia. 2001, 15: 1735-1742.View ArticlePubMedGoogle Scholar
- de la Lastra CA, Villegas I: Resveratrol as an antioxidant and pro-oxidant agent: mechanisms and clinical implications. Biochemical Society Transactions. 2007, 35: 1156-1160. 10.1042/BST0351156.View ArticlePubMedGoogle Scholar
- Commun K, Mauro MC, Chupeau Y, Boulay M, Burrus M, Jeandet P: Phytoalexin production in grapevine protoplasts during isolation and culture. Plant Physiology et Biochemistry. 2003, 41: 317-323. 10.1016/S0981-9428(03)00025-1.View ArticleGoogle Scholar
- Krisa S, Larronde F, Budzinski H, Decendit A, Deffieux G, Mérillon JM: Stilbene production by Vitis vinifera cell suspension cultures: methyl jasmonate induction and 13C biolabeling. Journal of Natural Products. 1999, 62: 1688-1690. 10.1021/np990239x.View ArticleGoogle Scholar
- Gundlach H, Muller MJ, Kutchan TM, Zenk MH: Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proceedings of the National Academy of Sciences of the United States of America. 1992, 89: 2389-2393. 10.1073/pnas.89.6.2389.PubMed CentralView ArticlePubMedGoogle Scholar
- Larronde F, Gaudillere JP, Krisa S, Decendit A, Deffieux G, Merillon JM: Airborne methyl jasmonate induces stilbene accumulation in leaves and berries of grapevine plants. American Journal of Enology and Viticulture. 2003, 54: 63-66.Google Scholar
- Repka V, Fischerová I, Šilhárová K: Methyl jasmonate is a potent elicitor of multiple defense responses in grapevine leaves and cell-suspension cultures. Biologia Plantarum. 2004, 48: 273-283. 10.1023/B:BIOP.0000033456.27521.e5.View ArticleGoogle Scholar
- Tassoni A, Fornale S, Franceschetti M, Musiani F, Michael AJ, Perry B, Bagni N: Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures. New Phytologist. 2005, 166: 895-905. 10.1111/j.1469-8137.2005.01383.x.View ArticlePubMedGoogle Scholar
- Morales M, Bru R, García-Carmona F, Ros Barceló A, Pedreño MA: Effect of dimethyl-β-cyclodextrins on resveratrol metabolism in Gamay grapevine cell cultures before and after inoculation with shape Xylophilus ampelinus. Plant Cell, Tissue and Organ Culture. 1998, 53: 179-187. 10.1023/A:1006027410575.View ArticleGoogle Scholar
- Bru R, Selles S, Casado-Vela J, Belchi-Navarro S, Pedreño MA: Modified cyclodextrins are chemically defined glucan inducers of defense responses in grapevine cell cultures. Journal of Agricultural and Food Chemistry. 2006, 54: 65-71. 10.1021/jf051485j.View ArticlePubMedGoogle Scholar
- Bru R, Pedreño MA: Method for the production of resveratrol in cell cultures. PCT Patent WO/2003/062406. 2003Google Scholar
- Kiselev KV, Dubrovina AS, Veselova MV, Bulgakov VP, Fedoreyev SA, Zhuravlev YN: The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells. Journal of Biotechnology. 2007, 128: 681-692. 10.1016/j.jbiotec.2006.11.008.View ArticlePubMedGoogle Scholar
- Saigne-Soulard C, Richard T, Mérillon JM, Monti JP: 13C NMR analysis of polyphenol biosynthesis in grape cells: Impact of various inducing factors. Analytica Chimica Acta. 2006, 563: 137-144. 10.1016/j.aca.2005.09.073.View ArticleGoogle Scholar
- Pauwels L, Morreel K, De Witte E, Lammertyn F, Van Montagu M, Boerjan W, Inze D, Goossens A: Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells. Proceedings of the National Academy of Sciences. 2008, 105: 1380-10.1073/pnas.0711203105.View ArticleGoogle Scholar
- Hall D, De Luca V: Mesocarp localization of a bi-functional resveratrol/hydroxycinnamic acid glucosyltransferase of Concord grape (Vitis labrusca). The Plant Journal. 2007, 49: 579-591. 10.1111/j.1365-313X.2006.02987.x.View ArticlePubMedGoogle Scholar
- Bru R, Lopez-Nicolas JM, Nunez-Delicado E, Nortes-Ruiperez D, Sanchez-Ferrer A, Garcia-Carmona F: Cyclodextrins as hosts for poorly water-soluble compounds in enzyme catalysis. Applied biochemistry and biotechnology. 1996, 61: 189-198. 10.1007/BF02785701.View ArticleGoogle Scholar
- Naill MC, Roberts SC: Cell cycle analysis of Taxus suspension cultures at the single cell level as an indicator of culture heterogeneity. Biotechnology and Bioengineering. 2005, 90: 491-500. 10.1002/bit.20446.View ArticlePubMedGoogle Scholar
- Naoumkina M, Farag MA, Sumner LW, Tang Y, Liu C-J, Dixon RA: Different mechanisms for phytoalexin induction by pathogen and wound signals in Medicago truncatula. Proceedings of the National Academy of Sciences. 2007, 104: 17909-17915. 10.1073/pnas.0708697104.View ArticleGoogle Scholar
- Zhao J, Davis LC, Verpoorte R: Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances. 2005, 23: 283-333. 10.1016/j.biotechadv.2005.01.003.View ArticlePubMedGoogle Scholar
- Calderon AA, Zapata JM, Munoz R, Pedreño MA, Barcelo AR: Resveratrol production as a part of the hypersensitive-like response of grapevine cells to an elicitor from Trichoderma viride. New Phytologist. 1993, 124: 455-463. 10.1111/j.1469-8137.1993.tb03836.x.View ArticleGoogle Scholar
- Dalluge JJ, Nelson BC, Thomas JB, Sander LC: Selection of column and gradient elution system for the separation of catechins in green tea using high-performance liquid chromatography. Journal of chromatography A. 1998, 793: 265-274. 10.1016/S0021-9673(97)00906-0.View ArticlePubMedGoogle Scholar
- Reid KE, Olsson N, Schlosser J, Peng F, Lund ST: An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biology. 2006, 6: 27-10.1186/1471-2229-6-27.PubMed CentralView ArticlePubMedGoogle Scholar
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