Figure 4

Northern blot analysis of expression of the Cu/ZnSOD gene in from D. antarctica. Changes in the levels of Cu/ZnSOD transcripts in D. antarctica plants subjected to cold (A) and UV (B) stress condition using total RNA extracted from leaves samples (Described in Methods). Equal aliquots (20 μg) of total RNA were electrophoresed in each lane, transferred onto nylon membrane and probed with ³²P-labeled gene specific DNA fragments (Left panels). Tubulin RNA probe was used as loading control on the same membranes. Desitometric analysis of the autoradiograms from three independent experiments (Right panels); n = 3 ± SE.