Table 1 Purification of recombinant SOD expressed by Pichia pastoris
Step | Volume (ml) | Protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) |
|---|---|---|---|---|---|
Supernatant | 45 | 11.25 | 900 | 80 | 100.0 |
Concentrated (1/5) | 9 | 6.40 | 134 | 21 | 14.9 |
ProBond | 8 | 0.26 | 113 | 440 | 12.5 |