Figure 2

Growth kinetics of rBoHV5 and rBoHV1 mutants versus wt BoHV5 and wt BoHV1. MDBK cells were infected at moi of 0.01 PFU with different viruses and harvested at various times post inoculation as indicated. The virus yields at each time point were determined by titration. Panels A and B: wt BoHV5 (full squares) and rBoHV5 (open squares). Panels C and D: wt BoHV1 (full circles) and rBoHV1 (open circles). Cell-free (supernatant, panels A and C) and cell-associated virus (pellets, panels B and D) were titrated separately. The x-axis represents the time scale post infection. Virus titers (y-axis) are expressed as TCID₅₀/ml.