Table 2 Primer Sequences and Combinations used in PCR-SSP-typing for Interleukin-6 Promoter -597/-572/-174Haplotype
Primermix | Primer Sequence | Product Size [bp] | - 597 Genotype | - 527 Genotype | - 174 Genotype | Detected major & (minor) Haplotypes | |
|---|---|---|---|---|---|---|---|
I | AS2-F3a AS8-R1g | 5'-tgaagtaactgcacgaaatttgagga-3' 5'-tgcaatgtgacgtcctttagcatg-3' | 473 | A | - | C | AGC (ACC) |
II | AS1-F1g AS8-R1g | 5'-aagtaactgcacgaaatttgaggg-3' 5'-tgcaatgtgacgtcctttagcatg-3' | 471 | G | - | C | GGC (GCC) |
III | AS3-F3g AS7-R1c | 5'-grtggccaggcagttctacaacagccg-3' 5'-tgcaatgtgacgtcctttagcatc-3' | 449 | - | G | G | GGG (AGG) |
IV | AS4-F3c AS7-R1c | 5'-grtggccaggcagttctacaacagccc-3' 5'-tgcaatgtgacgtcctttagcatc-3' | 449 | - | C | G | GCG (ACG) |
β-globF4 β-globR5 | 5'-gcttaccaagctgtgattcc-3' 5'-aaggtgcccttgaggttgtc-3' | 731 |