Figure 1From: KISSa: a strategy to build multiple sequence alignments from pairwise comparisons of very closely related sequencesUse of tags to create an alignment. To create this alignment, the query sequence read against the reference sequence until after amino acid #8 (reference sequence numbering), then a gap of 3 residues is inserted in the query (tag 8-3; deletion of length 3 in query). The sequences are continued until amino acid #21 (reference sequence numbering), then a gap of 1 residue is the reference sequence (tag 21g1; insertion of 1 amino acid in query sequence). There is no need for substitution tags in this example because both amino acid sequences have been stored.Back to article page