| Real Time PCR results |
---|
Conventional culture identification (n = 338) | Huletsky's[8] single-locus detectiona | Reischl's[5] double-locus detectionb | Combined resultsc |
---|
 |
SCC:orfX+
(MRSA) |
SCC:orfX-
(other) |
Sa442+ mec+
(MRSA) |
Sa442+ mec-
(MSSA) |
Sa442- mec+
(non S.A oxaR) |
Sa442- mec-
(other) | MRSAd | Not MRSAe |
---|
MRSA (n = 219) | 203 | 16 | 218 | 1 | --- | --- | 202 | 17f |
MSSA (n = 90) | 13 | 77 | (44+2)g | 35 | --- | 9 | 1h | 89i |
MR-CoNS (n = 16) | --- | 16 | --- | --- | 16 | --- | Â | 16 |
MS-CoNS (n = 8) | --- | 8 | --- | --- | --- | 8 | Â | 8 |
non-Staph. (n = 5) | --- | 5 | Â | Â | Â | 5 | Â | 5 |
- a sensitivity = 92.7%, specificity = 89.1%, Positive predictive value = 93.4%, Negative predicted value = 86.9%,
- b sensitivity = 99.5%, specificity = 61.3%, Positive predictive value = 82.6%, Negative predicted value = 98.6%
- c sensitivity = 92.2%, specificity = 99.2%, Positive predictive value = 99.5%, Negative predicted value = 87.4%
- d MRSA = SCC:orfX positive, Sa442 positive, mec positive
- e Not MRSA = all other results.
- f 16 SCC:orfX negative, Sa442 positive, mec positive and 1 oxacillin-resistant MSSA (SCC:orfX positive, Sa442 positive mec negative) classified as MRSA by culture methods.
- g 44 were MSSA mixed with MR-CoNS, 2 were oxacillin-susceptible MRSA classified as MSSA by culture methods.
- h 1 SCC:orfX positive, Sa442 positive, mec positive originated from a mixture of SCC:orfX positive, Sa442 positive, mec negative MSSA and mec positive MR-CoNS
- i 9 SCC:orfX negative, Sa442 negative, mec negative; 2 SCC:orfX negative, Sa442 positive, mec positive; 22 SCC:orfX negative, Sa442 positive, mec negative; 13 SCC:orfX positive, Sa442 positive, mec negative MSSA's and 43 SCC:orfX negative, Sa442 positive, mec