Criteria | Measure | Score values per criterion | |||
---|---|---|---|---|---|
0 | 1 | 4 | 20 | ||
CHI | % identity | 60% | 61- 84% | ≥ 85% | NAa |
Self-folding structure | ΔG | > -8 kcal/mol | ≤ -8 kcal/mol | NAa | NAa |
Probe position in CDS | Nucleotides numbered from CDS 3' end | 1-500 | 500 - 1000 | > 1000 | NAa |
Probe position relative to intron | Classes defined in Figure 1 | Class 1 | Class 2 | Class 3 | NAa |
Sequence match | Used after genome re-annotation | Perfect match | NAa | NAa | Mismatch |