Table 3 Primer sequences, purpose, annealing temperature (TA), number of bands and product size for primers during PCR, accession number and the linkage groups (LG) where the fragments were localized in Arctic charr
Primer Name | Primer Sequence (5'→3') |  | TA | # of Bands Band size | Sequence Name (GB Accession #) | Family & parent | LG |
|---|---|---|---|---|---|---|---|
Clock1a-1 | F-CCCAGTGTCCAGGTGAGAAA | Mapping | 57 |  |  | 3♀ | 16 |
| Â | R-CCTTTGTTCAGGGACACATT | Â | Â | Â | Â | Â | Â |
Clock1b | F-ACCCAGAATACAGGGCAGAAC | Sequencing | 51 | 2 | SalClock1b | Â | Â |
| Â | R-CAGGATCAATTGAGTGGACTGG | Â | Â | 1.5 kb, 1.3 kb | (GU228524) | Â | Â |
Clock1b-3 | F-GAGTACTGCCCTGCAGGTTG | Mapping | 55 |  |  | 3♂ | 13 |
| Â | R-TTGACCATGGCCCTCTTATG | Â | Â | Â | Â | Â | Â |
NPAS2-3 | F-CTTATAATCTCCACCCAGCACA | Mapping | 61 |  |  | 2♀ | 43 |
|  | R-GTCCGTGACAGAGTGCGATA |  |  |  |  | 3♂ | 20 |
amh | F-ACTCCTCCATGTGGTGCAA | Mapping |  |  |  | 2♀ | 13 |
|  | R-CCATACAGTCTTATCTGATCATGC |  |  |  |  | 3♀ | 13 |