Skip to main content
Figure 1 | BMC Research Notes

Figure 1

From: Use of RNAlater in fluorescence-activated cell sorting (FACS) reduces the fluorescence from GFP but not from DsRed

Figure 1

RNAlater decreases fluorescence of YFP. (A & B) FACS results. All data in FACS figures are restricted to cells defined by forward and side light scatter and further to singlet events. The YFP positive cells are shown enclosed by red lines and negative cells are shown enclosed by black lines. (A) Cells in BSA: a population of YFP positive cells is clearly distinguished from YFP negative cells. (B) Cells in RNAlater: the two populations are not discernable. (C & D) dissociated cells were observed under the fluorescence microscope (C) YFP positive cells in BSA. (D) YFP positive cells one minute after addition of RNAlater. The intensity of the YFP decreased substantially in the presence of RNAlater. (C & D) Exposure time: 200ms; scale bar: 50 microns.

Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us