Figure 1From: Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis in vivoComparison of current methods for total RNA isolation from the small cell tissue cartilage: quality control using capillary electrophoresis. Total RNA was isolated from chondrocytes (cells) and cartilage explants. Cartilage homogenization was performed with scalpel (SC), rotor-stator (RS) or microdismembrator (MD). Different extraction procedures (TRIzol®, RNeasy™, TRIzol®/RNeasy™ and RNAqueous™) were performed as described in the Methods section. Integrity of RNA isolated from different species (human, adult bovine cartilage - cow, and immature bovine cartilage - calf) was analyzed by capillary electrophoresis. 18S and 28S rRNA bands correspond to 41-43 and 47-50 [s], respectively. The RNA yield is specified for each sample [in ng/μl]. After precipitation and washing the RNA was resuspended in different volumes of RNase-free water: 30 μl (Trizol®), 20 μl (RNeasy™), 10 - 20 μl (Trizol®/RNeasy™) and 10 - 20 μl (RNAqueous™). On account of this, the results provided here are comparable with the results provided in Table 1 (μg RNA per 100 mg cartilage). The results are shown by gel electrophoresis (RIN: RNA integrity number; N/A: not available).Back to article page