Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress

Table 1 Primers for RTqPCR used in the present study.

Gene	Accesion #	Primer forward	Primer reverse	E.	Amplicon size
rplp1	EX741373	tccaaaccctaaaatccaaca	tggaggatcagagcagagtaaa	1,89	77
actb	AJ555463	acaccgtgcccatctacg	ccaagtccagacggaggat	2,05	60
b2m	acc386599	gagcccaacaccctgatct	gctcgatggtgatctctgg	2,11	61
ef1a	DQ402371	caggtcatcatcctgaacca	atccaggactggggcatag	2,02	60
g6pd	EX741924	acactctgcaccagggagtc	ccactgctgcaccacatc	1,88	98
tba1c	EL616694	ctccaccaggaactacagtgg	tagactggtgcccaactggt	2,10	69
ubiq	EX735613	cattgagccttccctcagaa	ttgcggcagatcatcttgt	1,98	63
18s	AJ427629	tgtgccgctagaggtgaaatt	gcaaatgctttcgctttcg	2,03	61
hsp70	ES478099	ggagttcaagcggaagttca	agcctcctcaaagccctct	1,87	60
hsp90	ES783928	tcctccgatactacacctcca	gcgagacacgtagtccttga	1,98	65

Reference and hsp's gene primer sequences, amplicon sizes, amplification efficiency values and accession # for the PCR analyses in the present study.

ISSN: 1756-0500