Comparison between nasopharyngeal swab and nasal wash, using culture and PCR, in the detection of potential respiratory pathogens
© Gritzfeld et al; licensee BioMed Central Ltd. 2010
Received: 22 November 2010
Accepted: 13 April 2011
Published: 13 April 2011
Nasopharyngeal carriage of potential pathogens is important as it is both the major source of transmission and the prerequisite of invasive disease. New methods for detecting carriage could improve comfort, accuracy and laboratory utility. The aims of this study were to compare the sensitivities of a nasopharyngeal swab (NPS) and a nasal wash (NW) in detecting potential respiratory pathogens in healthy adults using microbiological culture and PCR.
Healthy volunteers attended for nasal washing and brushing of the posterior nasopharynx. Conventional and real-time PCR were used to detect pneumococcus and meningococcus. Statistical differences between the two nasal sampling methods were determined using a nonparametric Mann-Whitney U test; differences between culture and PCR methods were determined using the McNemar test.
Nasal washing was more comfortable for volunteers than swabbing (n = 24). In detection by culture, the NW was significantly more likely to detect pathogens than the NPS (p < 0.00001). Overall, there was a low carriage rate of pathogens in this sample; no significant difference was seen in the detection of bacteria between culture and PCR methods.
Nasal washing and PCR may provide effective alternatives to nasopharyngeal swabbing and classical microbiology, respectively.
Bacterial colonization of the nasopharynx occurs early in life, with Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis causing sinusitis or otitis media in colonized children . Nasopharyngeal carriage of potential pathogens is important as it is both the major source of transmission and the prerequisite of invasive disease. Sampling techniques involved in the detection of nasopharyngeal colonization are quite varied. In adults, nasopharyngeal aspirate (NPA) cultures have been shown to be positive more often for pneumococci than oropharyngeal swab (OPS) cultures, but culture results were best when both types of specimens were taken . A similar study  found that nasopharyngeal methods (either swabs (NPS) or nasal washing (NW)) detected 89% of S. pneumoniae colonization as compared to 30% for OPS. There was no difference between the NPS and NW in detecting S. pneumoniae.
Detection of carriage is of great importance as it can be used in epidemiology studies, as an endpoint in vaccine trials, and in experimental human carriage projects, as has previously been done with S. pneumoniae. Limitations of detection in conventional microbiology have led to the development of PCR-based detection systems. The development of PCR-based serotyping systems could aid in the surveillance of vaccine-targeted serotypes and help overcome difficulties associated with serological testing.
The present study aimed to compare the sensitivity of a nasopharyngeal swab to a nasal wash in healthy adults using microbiological culture and molecular techniques (PCR).
Healthy adults aged 18-60 were recruited to the study, which was given ethical approval by the National Health Service Research Ethics Committee (08/H1001/52) and was sponsored by the Royal Liverpool and Broadgreen University Hospitals Trust. Both a NPS and NW were used to determine if a volunteer was carrying a potential pathogen in the nasopharynx. Volunteers positive for carriage of potential pathogens were asked to return for serial sampling.
The Borg Scale of Discomfort, kindly provided by Dr. Frederick Hargreave, McMaster University, was used to determine the level of discomfort caused by the NPS and NW (0 = no trouble at all, 10 = maximum discomfort, similar to congestion from a 'cold').
NPS specimens were vortexed in STGG medium and cultured on 7% horse blood agar and chocolate blood agar (Oxoid). NW specimens were centrifuged at high speed (1500 × g for seven minutes) and the pellet re-suspended in 1 ml STGG for storage; specimens were cultured on horse blood agar and chocolate blood agar. The remainder of the NPS and NW specimens were frozen at -80°C to be used for DNA extraction and PCR. All specimens were incubated for 24 hr at 37°C in 5% CO2.
Identification of Staphylococcus aureus was determined by catalase production and Pastorex Staph Plus latex test (Bio-Rad). S. pneumoniae was identified by colony morphology, alpha-haemolysis, optochin sensitivity, Gram stain, and bile solubility. M. catarrhalis was identified by colony morphology, Gram stain, a positive cytochrome oxidase test and hydrolysis of butyrate. Identification of Neisseria meningitidis was based on colony morphology, Gram stain, a positive cytochrome oxidase test and the API NH test strip (BioMérieux).
Verification of pneumococcal recovery from NW samples
To determine if S. pneumoniae could be recovered from NW specimens containing normal nasopharyngeal flora, five NW samples were spiked with 0 μl, 0.2 μl, 1 μl, 10 μl, and 100 μl of S. pneumoniae 23F stock culture. A viability count was performed on each spiked sample. The CFU of the starting culture was determined by a viability count and this was used to determine what concentration of S. pneumoniae 23F had been added to each vial.
DNA was extracted from the NPS and NW specimens using the QIAsymphony SP system and QIAsymphony Virus/Bacteria Midi Kit (QIAGEN Inc., UK) according to manufacturer's instructions. A total of 1200 μl was used in each reaction with an elution volume of 60 μl.
The conventional PCR assay for pneumococcus was taken from Pai et al. using only the cpsA primer pair. The final reaction volume was 20 μl and the assay was performed using BioMix Red (Bioline Ltd.), according to the manufacturer's instructions.
The real-time PCR assay for meningococcus [9, 10] was carried out in a final 25 μl reaction volume and was performed using TaqMan Universal Master Mix (Applied Biosystems) and 2.5 μl of sample DNA. DNA was amplified with the Corbett Rotor-Gene 6000 (QIAGEN) with the following cycling parameters: 50°C for 2 min, 95°C for 10 min, followed by 50 cycles of 15 s at 95°C and 1 min at 60°C. Amplification data were analyzed by instrument software (Rotor-Gene software series 1.7). Cycle threshold (Ct) values ≤35 were considered positive; Ct values between 36 and 40 were equivocal; and Ct values >40 were called negative. DNA extractions of equivocal specimens were diluted 1:4 and 1:10 and re-tested in duplicate. If the resulting average Ct values fell below 35, the specimen was considered positive. If the Ct values remained >35, the specimen was called negative.
Statistical analysis was performed using Microsoft Excel 2007, GraphPad Prism 4, and VassarStats (http://faculty.vassar.edu/lowry/propcorr.html, accessed 12-06-09). A distribution analysis was performed on data sets prior to examining statistical differences. A nonparametric Mann-Whitney U test was used to analyze the Borg Scale of Discomfort data. A McNemar test was used for the comparisons between NPS and NW. For all statistical comparisons a p value < 0.05 was defined as significant.
S. pneumoniae was not detected in any of the NPS or NW specimens but was detected in all four spiked NWs using the cpsA conventional PCR assay. The sodC real-time PCR assay detected N. meningitidis in NW more often than NPS but this result was not statistically significant (p < 0.25).
These results show that nasal washing is more comfortable than nasopharyngeal swabbing and is also more likely to detect potential pathogens. The Naclerio method of nasal washing has not been frequently used in the detection of bacteria; the most common method is a variation of instilling saline into the patient's nose and then suctioning it out through a tube, as was the method used by Lieberman et al.. The Naclerio method would not be recommended in children because of the level of participant cooperation required but in adults it was a far easier method which proved successful in this study in both degree of comfort and pathogen detection. Others have tried several techniques (anterior nares, throat swabs) to reduce sampling discomfort but it is important in pneumococcal work to sample the posterior nasopharynx. Our findings are similar to those by Lieberman et al. where NW was superior to NPS, especially in the detection of H. influenzae (p < 0.04).
The meningococcus real-time PCR is based on the detection of the sodC gene which encodes copper-zinc superoxide dismutase and is found in N. meningitidis, but not other Neisseria spp. [11, 12]. The sodC assay is 99.6% sensitive and 100% specific for detecting N. meningitidis. In this study, the only N. meningitidis culture positive sample was detected by real-time PCR, while Neisseria polysaccharea, Neisseria cinerea and M. catarrhalis were not detected using this assay. These results demonstrate that the sodC real-time PCR assay is sensitive and specific.
The combined NPS and NW results were examined but, due to the small sample size and the small number of volunteers found to be carrying N. meningitidis, the results were not significant. N. meningitidis was detected in only one culture sample and in two sodC PCR reactions. Only N. meningitidis could be analysed since S. pneumoniae was not detected by culture or conventional PCR.
There is currently an increased need for rapid detection of these potential pathogens, especially in relation to the recent H1N1 influenza A pandemic. In a group of U.S. patients with fatal pandemic influenza A, 29% showed evidence for a concurrent bacterial infection . S. pneumoniae was strongly correlated with disease when NPS samples taken from patients with confirmed H1N1 infection were examined with MassTag PCR . There is also an increased need for early recognition of bacterial pneumonia through easy-to-obtain samples and multiplex diagnostic methods. Current pneumococcal vaccine candidates are being designed to not only prevent pneumonia, but also prevent carriage and subsequently, transmission. Serial NW samples are a preferable sampling method to use in vaccine trials because of the increased detection capabilities and ease of administration as compared to NPS.
NW was chosen by 91% of volunteers as being more comfortable than NPS and was more likely to detect pathogens using microbiological culture. Detection of N. meningitidis appeared to be better by real-time PCR on NWs than on NPSs but further testing remains to be done to confirm this trend.
We would like to thank the Wellcome Trust, the National Institute for Health Research (NIHR), the NIHR Liverpool Biomedical Research Centre, Northwest Regional Development Agency (NWDA), the Bill and Melinda Gates Foundation, and Dr. Jennifer Dolan Thomas (Division of Bacterial Diseases, NCIRD, CDC) for providing the real-time PCR assay and technical support. Written consent for publication of the clinical photograph was obtained from the volunteer.
- Garcia-Rodriguez JA, Fresnadillo Martinez MJ: Dynamics of nasopharyngeal colonization by potential respiratory pathogens. J Antimicrob Chemother. 2002, 50 (Suppl S2): 59-73. 10.1093/jac/dkf506.PubMedView ArticleGoogle Scholar
- Watt JP, O'Brien KL, Katz S, Bronsdon MA, Elliott J, Dallas J, Perilla MJ, Reid R, Murrow L, Facklam R, et al: Nasopharyngeal versus oropharyngeal sampling for detection of pneumococcal carriage in adults. J Clin Microbiol. 2004, 42 (11): 4974-4976. 10.1128/JCM.42.11.4974-4976.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Lieberman D, Shleyfer E, Castel H, Terry A, Harman-Boehm I, Delgado J, Peled N: Nasopharyngeal versus oropharyngeal sampling for isolation of potential respiratory pathogens in adults. J Clin Microbiol. 2006, 44 (2): 525-528. 10.1128/JCM.44.2.525-528.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- McCool TL, Cate TR, Moy G, Weiser JN: The immune response to pneumococcal proteins during experimental human carriage. J Exp Med. 2002, 195 (3): 359-365. 10.1084/jem.20011576.PubMedPubMed CentralView ArticleGoogle Scholar
- Rubin LG, Rizvi A, Baer A: Effect of swab composition and use of swabs versus swab-containing skim milk-tryptone-glucose-glycerol (STGG) on culture- or PCR-based detection of Streptococcus pneumoniae in simulated and clinical respiratory specimens in STGG transport medium. J Clin Microbiol. 2008, 46 (8): 2635-2640. 10.1128/JCM.01903-07.PubMedPubMed CentralView ArticleGoogle Scholar
- O'Brien KL, Nohynek H: Report from a WHO Working Group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae. Pediatr Infect Dis J. 2003, 22 (2): e1-11.PubMedView ArticleGoogle Scholar
- Naclerio RM, Meier HL, Kagey-Sobotka A, Adkinson NF, Meyers DA, Norman PS, Lichtenstein LM: Mediator release after nasal airway challenge with allergen. Am Rev Respir Dis. 1983, 128 (4): 597-602.PubMedGoogle Scholar
- Pai R, Gertz RE, Beall B: Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol. 2006, 44 (1): 124-131. 10.1128/JCM.44.1.124-131.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Dolan J, Satterfield D, Team ABCSA, Mayer E: sodC as a species identifier for real-time PCR detection of. N. meningitidis. 16th International Pathogenic Neisseria Conference: 2008; Rotterdam, The Netherlands. 2008Google Scholar
- Dolan J, Hatcher C, Bach M, Satterfield D, Arnold KE, Stephens DS, Harrison LH, Hollick RA, Messonnier NR, Mayer LW, et al: Validation of a sodC-based real-time PCR assay for the detection of N. meningitidis. 10th European Meningococcal Disease Society Meeting: 2009; Manchester, UK. 2009Google Scholar
- Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW: GenBank. Nucleic Acids Res. 2009, D26-31. 10.1093/nar/gkn723. 37 Database
- Wilks KE, Dunn KL, Farrant JL, Reddin KM, Gorringe AR, Langford PR, Kroll JS: Periplasmic superoxide dismutase in meningococcal pathogenicity. Infect Immun. 1998, 66 (1): 213-217.PubMedPubMed CentralGoogle Scholar
- Thomas J, Hatcher C, Satterfield D, Theodore M, Bach M, Linscott K, Zhao X, Wang X, Mair R, Schmink S, et al: sodC-based Real-time PCR for Detection of Neisseria meningitidis. PLoS One. 2011,Google Scholar
- Louie J, Jean C, Chen TH, Park S, Ueki R, Harper T, Chmara E, Myers J, Stoppacher R, Catanese C, et al: Bacterial coinfections in lung tissue specimens from fatal cases of 2009 pandemic influenza A (H1N1) - United States, May-August 2009. MMWR Morb Mortal Wkly Rep. 2009, 58 (38): 1071-1074.Google Scholar
- Palacios G, Hornig M, Cisterna D, Savji N, Bussetti AV, Kapoor V, Hui J, Tokarz R, Briese T, Baumeister E, et al: Streptococcus pneumoniae coinfection is correlated with the severity of H1N1 pandemic influenza. PLoS One. 2009, 4 (12): e8540-10.1371/journal.pone.0008540.PubMedPubMed CentralView ArticleGoogle Scholar