Table 2 PCR conditions.
PCR No. | Initial denaturation | Denaturation | Annealing | Extension | Cycles | Final extension |
|---|---|---|---|---|---|---|
1 | 95°C, 15 min | 95°C, 30 s | 65°C, 30 s | 72°C, 1 min | 35 | 72°C, 10 min |
2 | 95°C, 15 min | 95°C, 30 s | 65°C, 30 s | 72°C, 1 min | 30 | 72°C, 10 min |
3 | 95°C, 15 min | 95°C, 30 s | 55°C, 30 s | 72°C, 1 min | 30 | 72°C, 10 min |
4 | 95°C, 15 min | 95°C, 30 s | 52°C, 30 s | 72°C, 1 min | 30 | 72°C, 10 min |