Archived Comments for:
Applying neutral drift to the directed molecular evolution of a β-glucuronidase into a β-galactosidase: Two different evolutionary pathways lead to the same variant
In table 1 the generation of two variant β-glucuronidases with increased β-galactosidase activity (labelled as C4.1 and C4.2) is described. Further analysis of the sequencing of these mutants has revealed a minor error in the listed mutation sites. Mutant C4.1 also contains a silent mutation. Mutant C4.2 is missing mutation sites N166T and N412D and the listing of a silent mutation. These make the mutants:
C4.1: G245A/K370R/W529L
C4.2: V67D/N166T/K370R/N412D/W529L
These changes do not affect the overall results and conclusions of our report.
Minor correction to sequence data
25 March 2014
In table 1 the generation of two variant β-glucuronidases with increased β-galactosidase activity (labelled as C4.1 and C4.2) is described. Further analysis of the sequencing of these mutants has revealed a minor error in the listed mutation sites. Mutant C4.1 also contains a silent mutation. Mutant C4.2 is missing mutation sites N166T and N412D and the listing of a silent mutation. These make the mutants:
C4.1: G245A/K370R/W529L
C4.2: V67D/N166T/K370R/N412D/W529L
These changes do not affect the overall results and conclusions of our report.
Cameron Neylon on behalf of the authors.
Competing interests
No competing interests.