Figure 3
From: The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
Detection of siRNA-mediated cleavage of RRM1 mRNA following in vitro transfection or intratumoural injection with RRM1 siRNAs. A549 xenograft tumours were injected as described in Figure 2 with RRM1-2 siRNA (A) or RRM1-3 siRNA (B). In vitro positive control: cDNA from A549 cells transfected with 1 nM RRM1-2 or RRM1-3 siRNA; samples were tested in triplicate, one single amplification curve is shown per sample for clarity.