Figure 4

Electropherogram of four SSR markers obtained by multiplexed post PCR labeling in a single tube. Genotyping of four SSR markers listed in Table 2 successfully gave individual peaks labeled with different fluorescent dyes in a single tube. 1) SSR08A04/F9GCC + VIC (green). 2) SSR08A09/F9GTC + PET (red). 3) SSR08B15/F9GAC + NED (black). 4) SSR08B25/F9TAC + 6-FAM (blue). Numbers under each peak represent estimated fragment size. These electropherograms were obtained for Binkitsu (C. platymamma hort. ex Tanaka).