Figure 1

Titration curve of G-CSF on the G-CSF dependant cell line NFS-60. NFS-60 were incubated with increasing concentrations of G-CSF for 48 h. The resulting viability curve was used to choose positive controls for the subsequent screen (0.1 nM and 0.1 pM G-CSF). The concentration of 0.1 nM (closed circle) corresponds to 100% viability of the cells. The concentration of 0.1 pM (dashed circle) reflects the lowest significant increase in viability observed.