Figure 1

Recognition of extract proteins from Trichoplusia ni insect larvae infected with recombinant baculoviruses BacNi (lanes A and E), G21-465 (lanes B and F), G21-507 (lanes C and G) and concentrated VHSV (lanes D and H) with anti polyHis (lanes A, B, C, D) or anti-gpG (lanes E, F, G, H) MAbs. Larvae were collected 72 hours after infection with the recombinant baculoviruses. Larvae were homogenized in guanidinium-free buffer and their soluble proteins were extracted. Thirty μg of protein extracts was loaded and electrophoresed in the same 12% polyacrylamide gel (PAGE) and transferred to a nitrocellulose membrane. The membrane was then cut and reacted with anti-His MAb (lanes A, B, C and D) or with anti-gpG MAb mix (lanes E, F, G and H) and peroxidase labelled anti-mouse Ig and then detected by chemiluminiscence. Some of the molecular weight markers on KDa are shown by the arrow numbers to the left and to the right.