An extended phylogenetic analysis reveals ancient origin of "non-green" phosphoribulokinase genes from two lineages of "green" secondary photosynthetic eukaryotes: Euglenophyta and Chlorarachniophyta
© Nozaki et al; licensee BioMed Central Ltd. 2011
Received: 22 March 2011
Accepted: 7 September 2011
Published: 7 September 2011
Euglenophyta and Chlorarachniophyta are groups of photosynthetic eukaryotes harboring secondary plastids of distinct green algal origins. Although previous phylogenetic analyses of genes encoding Calvin cycle enzymes demonstrated the presence of genes apparently not derived from green algal endosymbionts in the nuclear genomes of Euglena gracilis (Euglenophyta) and Bigelowiella natans (Chlorarachniophyta), the origins of these "non-green" genes in "green" secondary phototrophs were unclear due to the limited taxon sampling.
Here, we sequenced five new phosphoribulokinase (PRK) genes (from one euglenophyte, two chlorarachniophytes, and two glaucophytes) and performed an extended phylogenetic analysis of the genes based on a phylum-wide taxon sampling from various photosynthetic eukaryotes. Our phylogenetic analyses demonstrated that the PRK sequences form two genera of Euglenophyta formed a robust monophyletic group within a large clade including stramenopiles, haptophytes and a cryptophyte, and three genera of Chlorarachniophyta were placed within the red algal clade. These "non-green" affiliations were supported by the taxon-specific insertion/deletion sequences in the PRK alignment, especially between euglenophytes and stramenopiles. In addition, phylogenetic analysis of another Calvin cycle enzyme, plastid-targeted sedoheptulose-bisphosphatase (SBP), showed that the SBP sequences from two genera of Chlorarachniophyta were positioned within a red algal clade.
Our results suggest that PRK genes may have been transferred from a "stramenopile" ancestor to Euglenophyta and from a "red algal" ancestor to Chlorarachniophyta before radiation of extant taxa of these two "green" secondary phototrophs. The presence of two of key Calvin cycle enzymes, PRK and SBP, of red algal origins in Chlorarachniophyta indicate that the contribution of "non-green" algae to the plastid proteome in the "green" secondary phototrophs is more significant than ever thought. These "non-green" putative plastid-targeted enzymes from Chlorarachniophyta are likely to have originated from an ancestral red alga via horizontal gene transfer, or from a cryptic red algal endosymbiosis in the common ancestor of the extant chlorarachniophytes.
The Russian botanist Mereschkowski articulated the endosymbioitc theory a century ago . Today, most biologists believe that the endosymbiosis is responsible for the establishment of mitochondria and chloroplasts, which have played a critical role for the evolution of eukaryotes. Establishment of plastids is attributed to the process called primary endosymbiosis in which the host cell engulfed a cyanobacterial ancestor, and then some red and green algal ancestors were incorporated into other phagotrophic eukaryotes via secondary endosymbiosis and retained as secondary plastids . Almost all of the plastids in secondary and tertiary algae from stramenopiles, alveolates, haptophytes and cryptophytes (so-called 'chromalveolates') are thought to ultimately have originated from a secondary endosymbiosis of a red algal ancestor , whereas two eukaryotic groups, Euglenophyta and Chlorarachniophyta, possess secondary plastids of green algal origin . Recent studies demonstrated that the origins of the "green" secondary plastids of these two algal phyla are derived from independent secondary endosymbioses [4, 5].
In the process of primary and secondary endosymbioses, the genomic contents of the endosymbionts are reduced when compared to their presumed ancestors [2, 6] via gene loss and a process known as endosymbiotic gene transfer (EGT), in which a set of genes mostly assigned to the endosymbiont's functions is consequently moved to the nucleus of the host and merged into the chromosome [7–13]. EGT can be regarded as a special case of horizontal gene transfer (HGT), which is applicable to broader biological contexts such as a predacious or parasitic process [10, 14, 15]. Thus, extensive molecular phylogenetic analysis and careful examination of multiple possible HGT-derived genes are keys to give insights into historical events of endosymbioses (even including a cryptic endosymbiosis) in a eukaryotic cell .
In primary phototrophs, many of the nuclear-encoded genes encoding Calvin cycle (CC) enzymes are EGT-derived [11–13]. Phosphoribulokinase (PRK) (EC 220.127.116.11) is one of those CC enzymes, catalyzes conversion of ATP and D-ribulose 5-phosphate into ADP and D-ribulose 1,5-bisphosphate . Phylogenetic analysis suggested that PRK sequences are divided into two distantly related classes, Class I and Class II, which share approximately 20% amino acid (aa) identity [18, 19]. Proteobacterial Class I enzymes are octamers, whereas Class II enzymes from cyanobacteria and eukaryotic phototrophs function as tetramers and dimers, respectively . Although some CC genes are affiliated with non-cyanobacterial prokaryotic homologs [12, 13], the Class II PRK genes of the photosynthetic eukaryotes form a robust monophyletic group with cyanobacterial homologs, suggesting no gene replacement after the primary endosymbiosis (Additional file 1). In addition, PRK genes are relatively conserved among the CC genes . Thus, PRK may be an ideal gene to trace the historical events of endosymbioses of the plastids.
Phylogenetic analysis of PRK by Petersen et al.  shed light on the unusual origins of the genes from two lineages of "green" secondary phototrophs, Chlorarachniophyta and Euglenophyta. Bigelowiella natans (Chlorarachniophyta) has a "red alga-like" PRK gene while Euglena gracilis (Euglenophyta) has a "stramenopile-like" PRK. Obviously, unsolved problems remain because of only one chlorarachniophyte and one euglenophyte OTUs analyzed [13, 17]. A recent study on PRK phylogeny including several additional operational taxonomic units (OTUs) from green algae and dinoflagellates, in addition to taxon-specific insertion/deletion in the alignment, demonstrated strong affiliation between stramenopiles and Euglena likewise between Bigelowiella and red algae [21, 22]. However, each of the two green secondary phototrophic phyla still included only a single OTU, and the taxon samplings were also limited in the Glaucophyta and Chlorophyta (one of the two major clades of green plants or Viridiplantae) [13, 17, 21, 22].
The present study was undertaken to deduce the origins of "non-green" PRK genes from Euglenophyta and Chlorarachniophyta and to reconstruct more natural phylogenetic relationships of PRK from the major algal groups, employing a wider taxon sampling from various photosynthetic eukaryotes. We determined five new PRK genes from one euglenophyte, two chlorarachniophytes, and two glaucophytes and obtained several other PRK genes from the available genome and expressed sequence tag (EST) data up-to-date. Our extensive phylogenetic analyses of PRK genes demonstrated ancient origins of the "non-green" genes from the two algal groups harboring "green" secondary plastids (Euglenophyta and Chlorarachniophyta).
Strains and culture
The glaucophytes Gloeochaete wittrockiana SAG 46.84 and Glaucocystis nostochinearum SAG 16.98 were cultured in AF-6 medium  that was modified according to Kasai et al. . Eutreptiella gymnastica NIES-381 and Gymnochlora stellata CCMP 2057 were cultured in L1 medium  in which the natural seawater was replaced with Daigo's artificial seawater SP (Nihon Pharmaceutical Co. Ltd., Tokyo, Japan). The cultures were grown at 20°C with a 14 h: 10 h light: dark cycle. Chlorarachnion reptans NIES-624 was grown as described previously . All of these strains are unialgal, without contaminations of other algae.
RNA extraction and cDNA library construction
Cells of E. gymnastica, G. wittrockiana, and G. nostochinearum were crushed using ceramic beads and a Mixer Mill MM 300 (Qiagen, Hilden, Germany), and RNAs were subsequently extracted using the SV total RNA isolation system (Promega, Madison, WI, USA). Cells of G. stellata and C. reptans were disrupted and homogenized using brushes , and the RNA extraction was performed using the RNeasy Midi Kit (Qiagen). Reverse transcription (RT)-polymerase chain reaction (PCR) for all five RNA samples was performed using the Capfishing full-length cDNA Kit (Seegene, Seoul, Korea). The cDNAs were used as templates for PRK gene isolation.
Cloning and sequencing of phosphoribulokinase genes
For amplification of Class II PRK genes from cDNA, we designed degenerate primers based on conserved aa sequences of the published PRK protein sequences (Additional file 2). Nested PCR amplifications using these degenerate primers were carried out using the recombinant Taq™DNA polymerase (Takara Bio, Shiga, Japan). PCR was performed with 35 cycles at 95°C for 2 min, 46°C for 2 min, and 66°C for 3 min, followed by 72°C for 15 min using the Takara PCR Thermal Cycler (Takara Bio). First PCR products were amplified by PRK UF-1 and PRK UR-5, and the second were amplified by PRK UF-2 and PRK UR-4 (Additional file 2). Approximately 240 bp of PCR products were subsequently cloned into a plasmid vector (pCR®4-TOPO®) using a TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA, USA) for sequencing. Plasmids from positive clones were then sequenced using the BigDye™ Terminator v3.1 Cycle Sequencing Kit and the ABI PRISM 3100 genetic analyzer (Applied Biosystems, Foster City, CA, USA). Nucleotide sequences were determined based on at least three clones sharing the same sequence for each. Besides five new PRK sequences determined in the present study (Additional file 3), no other PRK sequences were obtained from the cloned PCR products, suggesting no contaminations of other algae during the experiment. Specific primers (Additional file 2) were designed using the partial sequences of PRK genes obtained from the cloned PCR products. A 3'-rapid amplification of cDNA ends (3'-RACE) was carried out using these specific primers, and the PCR products were sequenced by the direct sequencing method.
Most Class II PRK sequences were retrieved from the National Center for Biotechnology Information (NCBI) http://www.ncbi.nlm.nih.gov/ and Joint Genome Institute (JGI) http://www.jgi.doe.gov/. In this analysis, besides five new PRK sequences, one brown alga (Ectocarpus siliculosus), seven chlorophytes, and several other available sequences were added to OTUs used previously [17, 21]. Sequences of PRK genes from two charophycean algae, Closterium peracerosum-strigosum-littorale complex (Closterium psl complex) and Chara braunii, were obtained from unpublished assembled EST data (Nishiyama pers. comm.). The aa sequences of PRK from 42 eukaryotic ingroup and 14 cyanobacterial outgroup OTUs (including five genes sequenced in this study; Additional file 3) were aligned using SeaView , and ambiguous sites were removed from the alignment to produce a data matrix of 327 aa from 60 OTUs (available from TreeBase: http://www.treebase.org/treebase-web/home.html; study ID: s11802) (Additional file 4). All of the PRK nucleotide sequences used in the present study cover more than 300 aa within the 327 aa alignment except for EST database-retrieved sequences from the streptophyte Artemisia annua (230 aa), Beta vulgaris (262 aa), the glaucophyte Cyanophora paradoxa (153 aa), and the dinoflagellate Amphidinium carterae (292 aa). The following phylogenetic analyses were carried out, after excluding four dinoflagellate sequences that exhibit long branches and cause low phylogenetic resolution (Additional file 5).
Bayesian inference (BI) was conducted using MrBayes (ver. 3.1.2; ) with the WAG+I+Г4 model. BI consisted of two parallel runs with each of four Markov chain Monte Carlo (MCMC) incrementally heated chains and 1,000,000 generations, with sampling every 100 generations. The first 25% of the generations were discarded as burn-in, and the remaining trees were used to calculate a 50% majority-rule consensus tree and determine the posterior probabilities (PP) of the individual branches. The average standard deviation of split frequencies of the two MCMC iteration runs was below 0.01 for each analysis, indicating convergence. In addition, 1000 replicates of bootstrap analyses using the maximum likelihood (ML) method were performed using both RAxML (ver. 7.0.3; ) and PhyML (ver.3.0; ) with the WAG+I+Г4 model. Maximum parsimony (MP) analysis was also run with PAUP 4.0b10  with the nearest-neighbor-interchange search method to produce bootstrap values (BV) based on 1000 replicates.
In addition, we carried out two approximate unbiased tests (AU test)  to examine the phylogenetic positions of the two monophyletic groups of euglenophytes and chlorarachniophytes. We used two series of the phylogenetic trees of PRK sequences, where topologies of all the OTUs excluding either of the euglenophytes or chlorarachniophytes were fixed, and the alignment (327 aa) as input data. All possible topologies were generated by re-grafting the branch of euglenophytes or chlorarachniophytes using the in-house ruby script. The pools of topologies were analyzed with the AU test using the site-wise log-likelihood values were calculated using PhyML (with WAG model+F+I+Г4) and used for AU test conducted by Consel (ver. 0.1 k; ).
Analyses of sedoheptulose-bisphosphatase (SBP) genes were also carried out based on 275 aa from 37 OTUs (available from TreeBase: http://www.treebase.org/treebase-web/home.html; study ID: s11802) (Additional file 6) representing a wide range of eukaryotic organisms (including two chlorarachniophyte sequences) (Additional file 7) using the same phylogenetic methods as for the present PRK genes described above.
Programs for BI, ML and AU test were executed on a supercomputer (Human Genome Center, University of Tokyo, Japan).
The phylogenetic results of SBP sequences are shown in Additional file 8. As in the previous study , the SBP proteins were subdivided into two groups, one of which is composed of possible plastid-targeted proteins from green plants (Streptophyta and Chlorophyta), Euglena, four red algal sequences, Cyanophora, and two chlorarachniophytes (Bigelowiella and Gymnochlora). The two chlorarachniophytes and two red algae (Porphyra and Chondrus) formed a moderate monophyletic group, with 0.94 PP in BI and 61-69% BV in three other phylogenetic methods. Euglena was positioned within one of the chlorophyte lineages that constituted a monophyletic group (with 1.00 PP in BI and 65-81% BV in three other phylogenetic methods).
Many of the nuclear-encoded genes of CC enzymes are EGT-derived. But several CC enzymes are of non-cyanobacterial origin [12, 13]. Eukaryotic SBP is a nuclear-encoded gene of bacterial ancestry . Teich et al. argued that SBP genes found in phototrophic eukaryotes were likely to have originated from a single recruitment of plastid-targeted enzyme in photosynthetic eukaryotes after primary endosymbiosis and a further distribution to algae with secondary plastids via EGT . Our results are consistent with this scenario and we postulate that euglenophyte and chlorarachniophyte SBP genes were transferred from a green alga and a red alga, respectively.
Origin of the "non-green" genes from Chlorarachniophyta and its implication with secondary "green" plastids
Earlier phylogenetic analyses of the plastid-encoded genes and the nuclear-encoded plastid-targeted PsbO proteins demonstrated that the "green" secondary plastids of Chlorarachniophyta and Euglenophyta were of distinct origins, but the sequences from these two groups and green plants formed a robust monophyletic group as a whole [4, 5]. On the other hand, EST data of Bigelowiella natans showed the composition of nucleus genome is a mixture of genes derived from various sources . Consistent with previous studies [17, 21], our phylogenetic analysis of PRK proteins (Figure 1) and comparison of insertion/deletion sequences in the alignment (Figure 2) suggested that the three chlorarachniophyte PRK genes likely originated from non-green lineage, although the present AU test did not reject their green origin (Figure 3B). Based on the tree topology resolved (Figure 1), the most probable origin of the chlorarachniophyte PRK genes is a red algal ancestor. Several lines of research on the phylogeny of the Chlorarachniophyta [37–40] indicated that the three chlorarachniophyte genera examined in this study are distributed widely within this phylum. Therefore, it is likely that the PRK gene was transferred from an ancestral red alga before radiation of the extant taxa of Chlorarachniophyta.
Recent nuclear multigene phylogenetic studies of eukaryotes suggested that Rhizaria (including chlorarachniophytes) was a sister group to the clade composed of stramenopiles and alveolates [40–43]. However, the PRK protein phylogeny in this study showed that the clade composed of red algae and chlorarachniophytes is robustly separated from SHC group including stramenopiles (Figure 1). In addition, the AU test rejected the tree topology in which the chlorarachniophyte PRK genes were associated with stramenopile homologs (Figure 3B). Taking all these results together, it is unlikely that the chlorarachniophyte PRK genes were derived from the host component of Rhizaria.
Our phylogenetic analysis (Additional file 8) showed that at least two chlorarachniophyte sequences were nested within the red algae-derived SBP clade, which suggested a single HGT from an ancestral red alga to the ancestor of chlorarachniophytes. However, no insertion/deletion information was found in the SBP alignment for supporting the non-green origin of the genes (Additional file 6). A previous study proposed a hypothesis that the plastid-targeted SBP proteins of non-cyanobacterial origin was introduced and replaced the original cyanobacterial counterpart in the common ancestor of primary phototrophs, i.e., green plants, glaucophytes and red algae . As is the case with PR K, it is likely that the "red" SBP gene was acquired before the radiation of extant taxa of Chlorarachniophyta.
Archibald et al.  suggested that eight B. natans genes encoding plastid enzymes were derived from red algae or secondary algae harboring red algal plastids, and that these "red" lineage genes in B. natans were acquired via HGT through the feeding of red algal prey organisms by mixotrophic host chlorarachniophytes. Given this perspective, one possible explanation for the origin of the multiple red algal-derived CC genes in the chlorarachniophyte nuclear genomes is that these genes were transferred from red algal prey organisms via HGT. Furthermore, the fact that the essential functions of CC enzymes play key roles in the plastid metabolism is tempting to speculate that the red algal prey might have had a close interaction with the host and provided CC enzymes which accordingly enhanced photosynthetic performance. Alternatively, the prey organism might have been captured by and retained in an ancestral (and probably non-photosynthetic) chlorarachniophyte as an endosymbiont, which was then replaced by a green algal endosymbiont, giving rise to the extant secondary plastid in Chlorarachniophyta. Such a cryptic endosymbiosis scenario is consistent with the present consideration that the two CC enzymes (PRK and SBP) are likely originate from a red algal lineage before the radiation of the extant taxa of Chlorarachniophyta. To verify this hypothesis, we need to analyze as many genes as possible to find the consistent pattern among the gene trees.
"Non-green" origins of the PRK genes from Euglenophyta
The PRK sequences from the secondary phototrophic group Euglenophyta also showed the "non-green" affiliation in this analysis (Figure 1), despite the well-established notion that the euglenophyte plastids originated from green plants [4, 5]. Our results of AU test also did not support that the euglenophyte PRK genes originated from a basal lineage of the green plants or the prasinophyte-like secondary endosymbiont that gave rise to the secondary plastid in Euglenophyta (Figure 3A). Earlier phylogenetic research on nuclear genes [44–46] suggested that Euglena and Eutreptiella are representative genera of two major monophyletic groups in Euglenophyta. Our phylogenetic tree and comparison of insertion/deletion characters in the alignment demonstrated that PRK genes of these two genera are both stramenopile-like (Figures 1, 2), suggesting that the HGT of stramenopile PRK genes might have taken place before the radiation of the extant members of Euglenophyta. A recent study of putative stramenopile-derived genes in Euglena and Peranema (phagotrophic euglenoid), based on the single gene-based phylogenetic analysis using EST data, proposed a testable hypothesis on an ancient EGT from a stramenopile ancestor to the common ancestor of Euglenida (including both phototrophic and heterotrophic euglenoids) .
Origin of "green" PRK genes in stramenopiles
Our tree topology robustly resolved that green plants constitute a monophyletic group adjacent to SHC group as a sister group (Figure 1). Given the phylogenetic analyses of eukaryotes using slowly evolving nuclear genes suggesting that a large clade composed of stramenopiles and alveolates (and haptophytes) are sister to green plants [48, 49], the sister relationship of PRK genes between green plants and SHC group may have resulted from their host cell phylogeny. This implies that the ancestor of SHC group might once have been a photosynthetic alga harboring primary plastids which shared the same origin with green plants' counterparts [48–50]. Under this view, after the divergence between the SHC ancestor and green plants, the PRK genes within some lineages of SHC group might have been retained in the host nuclei even after the original "green" plastids were replaced by the extant "red" plastids via secondary endosymbiosis of a red alga .
Alternatively, Moustafa et al.  argued that an ancestor of stramenopiles and alveolates (and Rhizaria) might once have harbored a green algal endosymbiont. Besides the PRK gene, an expanded list of green-related genes has been reported in stramenopiles and alveolates [52, 53], which is consistent with the hypothesis on an EGT event from a green alga, possibly a mamiellalean ancestor (prasinophyte), in the ancestor of stramenopiles and alveolates . However, our PRK protein phylogeny resolved that SHC group was positioned outside the monophyletic green plants (including mamiellalean algae Ostreococcus and Micromonas) (Figure 1). Given such a phylogenetic position of SHC group, the "green" PRK genes in SHC group cannot be explained by the EGT from a mamiellalean ancestor.
The present phylogenetic results suggest that PRK genes may have been transferred from a "stramenopile" ancestor to Euglenophyta and from a "red algal" ancestor to Chlorarachniophyta before radiation of extant taxa of these two "green" secondary phototrophs. As discussed above, other stramenopile-like and red alga-like putative plastid-targeted enzymes are recognized in Euglenophyta and Chlorarachniophyta, respectively, allowing us to speculate a cryptic endosymbiosis of a non-green algal ancestor in each of the phyla . Thus, the contribution of "non-green" algae to the plastid proteome in the "green" secondary phototrophs is more significant than ever thought.
The super-computing resource was provided by Human Genome Center, Institute of Medical Science, University of Tokyo. Constructions of EST databases for Closterium and Chara were supported by a Grant-in-Aid for Scientific Research on Priority Areas "Comparative Genomics" (No. 20017013 to Dr. Tomoaki Nishiyama, Advanced Science Research Center, Kanazawa University). This work was supported by Grants-in-Aid for Research Fellowships for Young Scientists (No. 20-9894 to SM) and Scientific Research (No. 21657024 to HN; No. 22405014 to HirS and HN; No. 22770083 to HidS) from the Japan Society for the Promotion of Science, Japan; by Grants-in-Aid for Scientific Research on Innovative Areas "Elucidating Common Mechanisms of Allogeneic Authentication" (No. 22112521 to HirS and No. 22112505 to HN) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan; and by a Research Grant (to HidS) from the Hyogo Science and Technology Association, Hyogo, Japan.
- Mereschkowski C: Über Natur und Ursprung der Chromatophoren im Pflanzenreiche. Biol Centralbl. 1905, 25: 593-604.Google Scholar
- Hedges SB, Blair JE, Venturi ML, Shoe JL: A molecular timescale of eukaryote evolution and the rise of complex multicellular life. BMC Evol Biol. 2004, 4: 2-10.1186/1471-2148-4-2.PubMedPubMed CentralView ArticleGoogle Scholar
- Keeling PJ: The endosymbiotic origin, diversification and fate of plastids. Philos Biol Sci. 2010, 365: 729-748. 10.1098/rstb.2009.0103.View ArticleGoogle Scholar
- Rogers MB, Gilson PR, Su V, McFadden GI, Keeling PJ: The complete chloroplast genome of the chlorarachniophyte Bigelowiella natans: evidence for independent origins of chlorarachniophyte and euglenid secondary endosymbionts. Mol Biol Evol. 2007, 24: 54-62.PubMedView ArticleGoogle Scholar
- Takahashi F, Okabe Y, Nakada T, Sekimoto H, Ito M, Kataoka H, Nozaki H: Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J Phycol. 2007, 43: 1302-1309. 10.1111/j.1529-8817.2007.00411.x.View ArticleGoogle Scholar
- Futuyma DJ: On Darwin's shoulders. Natural History. 2005, 114: 64-68.Google Scholar
- Davis CC, Wurdack KJ: Host-to-parasite gene transfer in flowering plants: phylogenetic evidence from Malpighiales. Science. 2004, 305: 676-678. 10.1126/science.1100671.PubMedView ArticleGoogle Scholar
- Nickrent DL, Blarer A, Qiu YL, Vidal-Russell R, Anderson FE: Phylogenetic inference in Rafflesiales: the influence of rate heterogeneity and horizontal gene transfer. BMC Evol Biol. 2004, 4: 40-10.1186/1471-2148-4-40.PubMedPubMed CentralView ArticleGoogle Scholar
- Woloszynska M, Bocer T, Mackiewicz P, Janska H: A fragment of chloroplast DNA was transferred horizontally, probably from non-eudicots, to mitochondrial genome of Phaseolus. Plant Mol Biol. 2004, 56: 811-820. 10.1007/s11103-004-5183-y.PubMedView ArticleGoogle Scholar
- Li S, Nosenko T, Hackett JD, Bhattacharya D: Phylogenomic analysis identifies red algal genes of endosymbiotic origin in the chromalveolates. Mol Biol Evol. 2006, 23: 663-674.PubMedView ArticleGoogle Scholar
- Martin W, Schnarrenberger C: The evolution of the Calvin cycle from prokaryotic to eukaryotic chromosomes: a case study of functional redundancy in ancient pathways through endosymbiosis. Curr Genet. 1997, 32: 1-18. 10.1007/s002940050241.PubMedView ArticleGoogle Scholar
- Matsuzaki M, Misumi O, Shin-i T, Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Nishida K, Yoshida Y, Nishimura Y, Nakao S, Kobayashi T, Momoyama Y, Higashiyama T, Minoda A, Sano M, Nomoto H, Oishi K, Hayashi H, Ohta F, Nishizaka S, Haga S, Miura S, Morishita T, Kabeya Y, Terasawa K, Suzuki Y, Ishii Y, Asakawa S, Takano H, Ohta N, Kuroiwa H, Tanaka K, Shimizu N, Sugano S, Sato N, Nozaki H, Ogasawara N, Kohara Y, Kuroiwa T: Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature. 2004, 428: 653-657. 10.1038/nature02398.PubMedView ArticleGoogle Scholar
- Reyes-Prieto A, Bhattacharya D: Phylogeny of Calvin cycle enzymes supports Plantae monophyly. Mol Phylogenet Evol. 2007, 45: 384-391. 10.1016/j.ympev.2007.02.026.PubMedView ArticleGoogle Scholar
- Hall C, Brachat S, Dietrich FS: Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae. Eukaryotic Cell. 2005, 4: 1102-1115. 10.1128/EC.4.6.1102-1115.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Kondo N, Nikoh N, Ijichi N, Shimada M, Fukatsu T: Genome fragment of Wolbachia endosymbiont transferred to × chromosome of host insect. Proc Natl Acad Sci USA. 2002, 99: 14280-14285. 10.1073/pnas.222228199.PubMedPubMed CentralView ArticleGoogle Scholar
- Stiller JW, Huang J, Ding Q, Tian J, Goodwillie C: Are algal genes in nonphotosynthetic protists evidence of historical plastid endosymbioses?. BMC Genomics. 2009, 10: 484-10.1186/1471-2164-10-484.PubMedPubMed CentralView ArticleGoogle Scholar
- Petersen J, Teich R, Brinkmann H, Cerff R: A "green" phosphoribulokinase in complex algae with red plastids: evidence for a single secondary endosymbiosis leading to haptophytes, cryptophytes, heterokonts, and dinoflagellates. J Mol Evol. 2006, 62: 143-157. 10.1007/s00239-004-0305-3.PubMedView ArticleGoogle Scholar
- Tabita FR: The biochemistry and molecular regulation of carbon dioxide metabolism in cyanobacteria. The Molecular Biology of Cyanobacteria. Edited by: Bryant DA. 1994, Dordrecht: Kluwer, 437-467.View ArticleGoogle Scholar
- Brandes HK, Hartmann FC, Lu T-Y, Larimer FW: Efficient expression of the gene for spinach phosphoribulokinase in Pichia pastoris and utilization of the recombinant enzyme to explore the role of regulatory cysteinyl residue by site-directed mutagenesis. J Biol Chem. 1996, 271: 6490-6496. 10.1074/jbc.271.11.6490.PubMedView ArticleGoogle Scholar
- Harrison DH, Runquist JA, Holub A, Miziorko HM: The crystal structure of phosphoribulokinase from Rhodobacter sphaeroides reveals a fold similar to that of adenylate kinase. Biochemistry. 1998, 37: 5074-5085. 10.1021/bi972805y.PubMedView ArticleGoogle Scholar
- Minge MA, Shalchian-Tabrizi K, Tørresen OK, Takishita K, Probert I, Inagaki Y, Klaveness D, Jakobsen KS: A phylogenetic mosaic plastid proteome and unusual plastid-targeting signals in the green-colored dinoflagellate Lepidodinium chlorophorum. BMC Evol Biol. 2010, 10: 191-10.1186/1471-2148-10-191.PubMedPubMed CentralView ArticleGoogle Scholar
- Rumpho ME, Pochareddy S, Worful JM, Summer EJ, Bhattacharya D, Pelletreau KN, Tyler MS, Lee J, Manhart JR, Soule KM: Molecular characterization of the Calvin cycle enzyme phosphoribulokinase in the stramenopile alga Vaucheria litorea and the plastid hosting mollusc Elysia chlorotica. Mol Plant. 2009, 2: 1384-1396. 10.1093/mp/ssp085.PubMedPubMed CentralView ArticleGoogle Scholar
- Kato S: Laboratory culture and morphology of Colacium vesiculosum Ehrb. (Euglenophyceae). Jpn J Phycol. 1982, 30: 63-67.Google Scholar
- Kasai F, Kawachi M, Erata M, Mori F, Yumoto K, Sato M, Ishimoto M: NIES-Collection List of Strains. Jpn J Phycol. 2009, 57 (Suppl 1): 1-350. 8Google Scholar
- Guillard RRL, Hargraves PE: Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia. 1993, 32: 234-236. 10.2216/i0031-8884-32-3-234.1.View ArticleGoogle Scholar
- Nozaki H, Ito M, Watanabe MM, Takano H, Kuroiwa T: Phylogenetic analysis of morphological species of Carteria (Volvocales, Chlorophyta) based on rbcL gene sequences. J Phycol. 1997, 33: 864-867. 10.1111/j.0022-3646.1997.00864.x.View ArticleGoogle Scholar
- Gouy M, Guindon S, Gascuel O: SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol. 2010, 27: 221-224. 10.1093/molbev/msp259.PubMedView ArticleGoogle Scholar
- Huelsenbeck JP, Ronquist F: MRBAYES: Bayesian inference of phylogeny. Bioinformatics. 2001, 17: 754-755. 10.1093/bioinformatics/17.8.754.PubMedView ArticleGoogle Scholar
- Stamatakis A: RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006, 22: 2688-2690. 10.1093/bioinformatics/btl446.PubMedView ArticleGoogle Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704. 10.1080/10635150390235520.PubMedView ArticleGoogle Scholar
- Swofford DL: PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Version 4. 2002, Sunderland: Sinauer AssociatesGoogle Scholar
- Shimodaira H: An approximately unbiased test of phylogenetic tree selection. Syst Biol. 2002, 51: 492-508. 10.1080/10635150290069913.PubMedView ArticleGoogle Scholar
- Shimodaira H, Hasegawa M: CONSEL: for assessing the confidence of phylogenetic tree selection. Bioinformatics. 2010, 17: 1246-1247.View ArticleGoogle Scholar
- Teich R, Zauner S, Baurain D, Brinkmann H, Petersen J: Origin and distribution of Calvin cycle fructose and sedoheptulose bisphosphatases in Plantae and complex algae: a single secondary origin of complex red plastids and subsequent propagation via tertiary endosymbioses. Protist. 2007, 158: 263-276. 10.1016/j.protis.2006.12.004.PubMedView ArticleGoogle Scholar
- Martin W, Mustafa AZ, Henze K, Schnarrenberger C: Higher-plant chloroplast and cytosolic fructose-1, 6-bisphosphatase isoenzymes: origins via duplication rather than prokaryote-eukaryote divergence. Plant Mol Biol. 1996, 32: 485-491. 10.1007/BF00019100.PubMedView ArticleGoogle Scholar
- Arichbald JM, Rogers MB, Toop M, Ishida K, Keeling PJ: Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga Bigelowiella natans. PNAS. 2003, 100: 7678-7683. 10.1073/pnas.1230951100.View ArticleGoogle Scholar
- Ishida K, Green BR, Cavalier-Smith T: Diversification of a chimaeric algal group, the chlorarachniophytes: phylogeny of nuclear and nucleomorph small-subunit r RNA genes. Mol Bio Evol. 1999, 16: 321-331.View ArticleGoogle Scholar
- Archibald JM, Keeling PJ: Actin and ubiquitin protein sequences support a cercozoa/floraminiferan ancestry for the plasmodiophorid plant pathogens. J Eukaryot Microbiol. 2004, 51: 113-118. 10.1111/j.1550-7408.2004.tb00172.x.PubMedView ArticleGoogle Scholar
- Chantangsi C, Hoppenrath M, Leander BS: Evolutionary relationships among marine cercozoans as inferred from combined SSU and LSU r DNA sequences and polyubiquitin insertions. Mol Phylogenet Evol. 2010, 57: 518-527. 10.1016/j.ympev.2010.07.007.PubMedView ArticleGoogle Scholar
- Parfrey LW, Grant J, Tekle YI, Lasek-Nesselquist E, Morrison HG, Sogin ML, Patterson DJ, Katz LA: Broadly sampled multigene analyses yield a well-resolved eukaryotic tree of life. Syst Biol. 2010, 59: 518-533. 10.1093/sysbio/syq037.PubMedPubMed CentralView ArticleGoogle Scholar
- Hackett JD, Yoon HS, Li S, Reyes-Prieto A, Rummele SE, Bhattacharya D: Phylogenomic analysis supports the monophyly of cryptophytes and haptophytes and the association of Rhizaria with chromalveolates. Mol Biol Evol. 2007, 24: 1702-1713. 10.1093/molbev/msm089.PubMedView ArticleGoogle Scholar
- Burki F, Inagaki Y, Bråte J, Archibald JM, Keeling PJ, Cavalier-Smith T, Sakaguchi M, Hashimoto T, Horak A, Kumar S, Klaveness D, Jakobsen KS, Pawlowski J, Shalchian-Tabrizi K: Large-scale phylogenomic analyses reveal that two enigmatic protist lineages, Telonemia and Centroheliozoa, are related to photosynthetic chromalveolates. Genome Biol Evol. 2009, 1: 231-238.PubMedPubMed CentralView ArticleGoogle Scholar
- Bodył A, Stiller JW, Mackiewicz P: Chromalveolate plastids: direct descent or multiple endosymbioses?. Trends Ecol Evol. 2009, 24: 119-121. 10.1016/j.tree.2008.11.003.PubMedView ArticleGoogle Scholar
- Müllner AN, Angeler DG, Samuel R, Linton EW, Triemer RE: Phylogenetic analysis of phagotrophic phototrophic and osmotrophic euglenoids by using the nuclear 18S r DNA sequence. IJSEM. 2010, 51: 783-791.Google Scholar
- Leander BS: Did trypanosomatid parasites have photosynthetic ancestors?. Trends Microbiol. 2004, 12: 251-258. 10.1016/j.tim.2004.04.001.PubMedView ArticleGoogle Scholar
- Linton EW, Karnkowska-Ishikawa A, Kim JI, Shin W, Bennett MS, Kwiatowski J, Zakryś B, Triemer RE: Reconstructing euglenoid evolutionary relationships using three genes: nuclear SSU and LSU, and chloroplast SSU r DNA sequences and the description of Euglenaria gen. nov. (Euglenophyta). Protist. 2010, 161: 603-619. 10.1016/j.protis.2010.02.002.PubMedView ArticleGoogle Scholar
- Maruyama S, Suzaki T, Weber AP, Archibald JM, Nozaki H: Eukaryote-to-eukaryote gene transfer gives rise to genome mosaicism in euglenids. BMC Evol Biol. 2011, 11: 105-10.1186/1471-2148-11-105.PubMedPubMed CentralView ArticleGoogle Scholar
- Nozaki H, Iseki M, Hasegawa M, Misawa K, Nakada T, Sasaki N, Watanabe M: Phylogeny of primary photosynthetic eukaryotes as deduced from slowly evolving nuclear genes. Mol Bio Evol. 2007, 24: 1592-1595. 10.1093/molbev/msm091.View ArticleGoogle Scholar
- Nozaki H, Maruyama S, Matsuzaki M, Nakada T, Kato S, Misawa K: Phylogenetic positions of Glaucophyta, green plants (Archaeplastida) and Haptophyta (Chromalveolata) as deduced from slowly evolving nuclear genes. Mol Phylog Evol. 2009, 53: 872-880. 10.1016/j.ympev.2009.08.015.View ArticleGoogle Scholar
- Matsuzaki M, Kuroiwa H, Kuroiwa T, Kita K, Nozaki H: A cryptic algal group unveiled: a plastid biosynthesis pathway in the oyster parasite Perkinsus marinus. Mol Biol Evol. 2008, 25: 1167-1179. 10.1093/molbev/msn064.PubMedView ArticleGoogle Scholar
- Moustafa A, Beszteri B, Maier UG, Bowler C, Valentin K, Bhattacharya D: Genomic footprints of a cryptic plastid endosymbiosis in diatoms. Science. 2009, 324: 1724-1726. 10.1126/science.1172983.PubMedView ArticleGoogle Scholar
- Frommolt R, Werner S, Paulsen H, Goss R, Wilhelm C, Zauner S, Maier UG, Grossman AR, Bhattacharya D, Lohr M: Ancient recruitment by chromists of green algal genes encoding enzymes for carotenoid biosynthesis. Mol Biol Evol. 2008, 25: 2653-2667. 10.1093/molbev/msn206.PubMedView ArticleGoogle Scholar
- Huang J, Gogarten JP: Did an ancient chlamydial endosymbiosis facilitate the establishment of primary plastids?. Genome Biol. 2008, 8: R99-View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.