Figure 3From: Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencingNumber of commonly or statistically differentially regulated genes in wild type and aak-2 mutants in response to oxidative stress and examples. (A) Venn diagram showing numbers of commonly up or (B) down-regulated genes with statistically significant gene expression level changes in wild type and aak-2 mutants in response to oxidative stress relative to wild type (overlapping regions) as well as numbers of genes up or down-regulated exclusively in stressed wild type or stressed aak-2 mutants relative to wild type (non-overlapping regions). Numbers in brackets indicate genes that showed the most difference in gene expression relative to wild type between the two libraries, stressed wild type and stressed aak-2 (i.e. genes that showed significant changes in one library (i.e. Log2 ratio relative to wild type greater or less than |0.7|, p-val < 0.01), but showed negligible changes (i.e. Log2 ratio relative to wild type greater or less than ~|0.3|) or significantly changed in the opposite direction in the other library). For instance, there is a relatively large subset of genes in brackets (125) significantly up-regulated in aak-2 mutants in response to oxidative stress but either insignificantly regulated or significantly down-regulated in wild type under oxidative stress. (C) UCSC browser view of hsp-16.2 and hsp-16.41 with the sequence reads aligned to the gene models. Four samples (Stressed wild type (N2), Stressed aak-2, Unstressed wild type (N2), Unstressed aak-2) are shown. It is evident hsp-16.2 and hsp-16.41 are much more highly expressed in stressed wild type and stressed aak-2 mutants compared to unstressed wild type and unstressed aak-2 mutants, especially in stressed aak-2 mutants. Note: The scales for the minimum and maximum number of reads for the four samples are different. (D) UCSC browser view of fat-7 with the sequence reads aligned to the gene model. Four samples (Stressed wild type, Stressed aak-2, Unstressed wild type, Unstressed aak-2) are shown. As indicated by the number of sequence reads mapped, fat-7 is much more highly expressed in aak-2 mutants (stressed and unstressed) compared to wild type (stressed and unstressed). Note: The scales for the minimum and maximum number of reads for the four samples are different.Back to article page