Figure 1

Short circuit current (I _sc ) across primary cultures of semicircular canal duct (SCCD) epithelial cells grown on permeable supports. A) Representative traces. Basolateral solution was physiological saline and apical solution was either Na⁺-rich (left) or K⁺-rich (right). Note break in time axis; Isc of each culture insert was measured in each apical solution using different order of exposure and washing in between measurements. Amiloride (100 μM) added on the apical side as indicated by horizontal red bar. B) Summary of normalized currents from experiments as in A.