Figure 3From: Unveiling novel genes upregulated by both rhBMP2 and rhBMP7 during early osteoblastic transdifferentiation of C2C12 cellsRelative expression levels of genes related to the extracellular matrix in C2C12 cells treated with rhBMP2 and rhBMP7. Cells were seeded and grown for 24 h at which point the medium was replaced by DMEM containing 5% FCS in the presence or absence of rhBMP2 (200 ng/ml) or rhBMP7 (200 ng/ml). RNA was isolated 0, 4, 8, 12 and 24h after stimulation. Subsequently, expression levels were measured by quantitative real time RT-PCR. The asterisks correspond to the statistical analysis (two-way ANOVA, Bonferroni test) between control and treatment (*p < 0.05; **p < 0.01; ***p < 0.001).Back to article page