Visceral leishmaniasis in 26 HIV-negative adults
© Lahlou et al; licensee BioMed Central Ltd. 2011
Received: 10 February 2011
Accepted: 8 October 2011
Published: 8 October 2011
Visceral leishmaniasis is a notifiable parasitic disease that had increased in incidence in our region on the past few years. It is common in children. In adults, it occurs more on a background of immunodeficiency, and frequently with incomplete clinical manifestations, making the diagnosis complicated.
The aim of our study is to reveal different features of visceral leishmaniasis in adults, through the analysis of its epidemiological, clinical and biological parameters, in a group of 26 patients. No one was infected with HIV or under immunosuppressive therapy Clinical presentation was generally conservative, but there was few differences in adults compared to children, concerning both the clinical symptoms and the laboratory parameters. Diagnosis was provided by direct examination of bone marrow smears in 24 cases (sensitivity 92%), and anti-leishmanial serology in the others.
We should think to the diagnosis of VL even if the patient is not known immunocompromised, and even if the clinical is incomplete, to avoid a delay of care which can lead to serious complications.
Keywordsvisceral leishmaniasis HIV-negative adults bone marrow aspirate immunofluorescent antibody test
Visceral leishmaniasis (VL), also known as kala-azar, is a systemic parasitic infection caused by two of the species of the protozoan Leishmania : L. donovani and L. Infantum. It is spread by the bite of the female sand fly Phlebotomus. It affects approximately 500.000 people every year [2–4], and almost 1000 individuals in the Mediterranean basin .
Its typical presentation associate fever, splenomegaly, hepatomegaly, lymph node enlargement and pancytopenia.
Conventional laboratory techniques for the diagnosis of VL are parasitological methods, based on the search for parasites on bone marrow smears or other tissues, and in vitro cultivation, and serological methods, favoured by the presence of serum antibodies; the most commonly used are immunofluorescent antibody test (IFAT) and immunoenzymatic methods (ELISA). PCR assays are also proposed as useful tools for the diagnosis of VL, through the detection of Leishmania DNA in different biological samples such as peripheral blood and bone marrow aspirate [1, 4].
Through this retrospective analysis, we try to discuss the specifics of VL in immunocompetent adults. Patients included are beyond 15 years-old, collected in the internal medicine department at the University Hospital of Fez, between June 2003 and August 2010. All of them were not infected with HIV, and they all underwent a bone marrow aspiration and a serological test of leishmaniasis based on immunofluorescent antibody test (IFAT) for the diagnosis.
We collected 26 cases of visceral leishmaniasis diagnosed in HIV-negative adults. Patients ranged in age from 15 to 65 years, with mean ages of 28 years. There were 15 men and 11 women. All patients were from regions already known to be endemic foci of VL, 61% of them were from rural areas, mainly from the vicinity of Fez and Taounate wich are cities in the center of Morocco.
Two patients were diabetic, one had chronic hepatitis C, another suffered from chronic renal failure and in two cases, VL was associated with pregnancy. We have also noted the particular association of VL with pernicious anemia in a woman, and with a spondylarthropathy in an adolescent.
The average consultation time was 107 days. More than half of our patients had consulted the first time for abdominal symptoms. In other cases, hematological and general symptoms were inaugural.
Clinical characteristics of the 26 patients
No. of patients
Edema of lower limbs +/- ascites
Biological characteristics of the 26 patients
White blood cells (cells/mm3)
Total protein (g/L)
An anti-HIV serology was performed in all patients and was negative. It was the same for the serology for hepatitis B and syphilis, made in 15 cases. One patient was positive for Hepatitis C tests.
All patients were treated with injections of antimoniate of Méglumine (Glucantime®). They received a single cure of 21 days at a dose of 80 mg/kg/day, administered gradually. This treatment was generally well tolerated. We have noted a single case of liver toxicity that resolved after dose reduction of Glucantime®. There was no relapse in our series.
The outcome was favorable in 23 cases (88%), with a progressive improvement in clinical parameters with obtaining apyrexia, disappearing of splenomegaly and improving general condition, then in biological assessments with decreasing of the inflammatory syndrome and normalization of the liver enzymes levels. No infectious or hemorrhagic complications were recorded. Although, the outcome in both pregnant women was characterized by the occurrence of portal hypertension in one, and fetal death in the other. We recorded an unexplained death of a patient of 60 years-old with renal failure, on the 4th day of treatment.
Epidemiological and etiopathogenic features
During the last years, a modification of the epidemiological profile of VL was noted. This disease is, up to now, quite rare among adults, became increasingly common, especially among immunocompromised individuals. In North-Mediterranean countries for example, there was an inversion of VL case distribution according to age [6, 7], in relation to the spread of HIV infection mainly, and also to the use of immunosuppressive therapies, such as long-term corticosteroid treatments  and immunosuppressants in systemic diseases, chemotherapy for cancer, and transplant anti-rejection therapy in allograft patients . Any condition able to weaken immune defenses of the host represents a fertile background for the development of VL. This infection is therefore, currently considered as an opportunistic disease. Indeed, several factors have been reported by various authors in literature [10–13], namely diabetes, terminal chronic renal failure, liver cirrhosis, tuberculosis, cancer, and also pregnancy on account of hormonal and immune changes which occur in pregnant women .
Nevertheless, several cases of VL were diagnosed in apparently immunocompetent adults, especially in North Africa, with significant proportions in most series. They were around 57%, 45% and 40% according to Afrit et al , Aoun et al  and Hakem et al  respectively.
In our series, the HIV serology was negative each time it was performed. None of our patients had received immunosuppressive therapy, and only 8 cases of VL had occurred on weakened immune terrain (diabetes, pregnancy, renal failure, hepatitis C, megaloblastic anemia and spondylarthropathy). Probably and for the others, it's possible that virulent parasite strains have been selected and may induce disorders even in adults .
Clinical manifestations of VL in adults are sometimes discreet and are not always suggestive. Moreover, even when a febrile hepatosplenic pancytopenic syndrome is present, diagnosis is often delayed because those signs are common to several other diseases  whose incidence is higher in adults.
Comparison of different VL series in adults
(n = 6)
(n = 18)
(n = 7)
(n = 64)
(n = 26)
Anti-Leishmania serology (+)
Amastigotes on bone marrow
Nonspecific biological signs
Hemogram, erythrocyte sedimentation rate and electrophoresis of plasmatic proteins are the main tests in the diagnosis process in VL. They show non-specific disorders such the common anemia which is normochromic, normocytic and non-regenerative. First moderate, then it gets progressively worse during the evolution, but not as fast as in children. Leukopenia, which was absent in 24% of our patients is, according to other authors, regularly observed . Thrombocytopenia is often delayed or may even miss , but late in disease progression, it is sometimes associated with alteration in the synthesis of coagulation factors by the liver, causing bleeding which can be serious or even fatal.
Inflammation is very marked; in fact, ESR that usually reaches 50-80 mm the first hour in children , is even higher among adults (99 mm in our series). CRP is also greatly increased.
Total serum protein is highly variable; it may be normal or high, particularly in adults. However, electrophoresis of proteins is very disturbed almost steadily. It shows a polyclonal hypergammaglobulinemia affecting mainly IgG, with hypoalbuminemia .
Specific biological signs
As clinical presentation of VL and hemato-biochemical disruption are unspecific, the detection of serum antibodies by serology, or better yet, the visualization of the parasite in any involved organ, are required for the diagnosis .
The anti-Leishmania antibodies are detected routinely by direct immunofluorescence technique, ELISA or indirect hemagglutination, and currently by immunoblotting on nitrocellulose membrane [25, 26]. Serology can be negative, despite the wealth of the bone marrow in Leishmania, especially in immunodeficient patients .
PCR also can be used; it may detect Leishmania DNA in different biological samples such as peripheral blood and bone marrow aspirate .
Concerning parasitological methods, the search for parasites must be first in the bone marrow, and an initial failure does not challenge the diagnosis. In immunocompromised patients, we can even find Leishmania on the peripheral blood after leukoconcentration.
In vitro cultivation has a good sensitivity but is carried out only in specialized centers, and if is negative or if the parasites are scanty, the results can be obtained only after several weeks.
In our series, the identification of the parasite by direct examination of the bone marrow allowed to the diagnosis in 24 patients, which means a sensitivity of 92%, comparable to that reported in the literature [27–29]. For the 2 patients whose bone marrow was negative, the diagnosis of VL was selected on the results of serology and clinical manifestations.
VL affects more and more adults in the Mediterranean area. This means that special care must be given to diagnosis, especially since clinical signs are often incomplete and little characteristic.
Thus, the disease should be suspected whenever there is prolonged or unexplained fever, splenomegaly or hypergammaglobulinemia in a patient from an endemic area, even if he is not infected with HIV or receiving immunosuppressive therapy. And when VL is diagnosed in those adults, an underlying condition that could weaken the immunity of the patient must be seeked, but even if there is not, this does not rule out the diagnosis of VL.
all the staff of the Department of Internal Medicine and Laboratory of Hematology.
- Chappuis F, Sundar S, Hailu A, et al: Visceral leishmaniasis: what are the needs for diagnosis, treatment and control?. Nat Rev Microbiol. 2007, 5: 873-882.PubMedView ArticleGoogle Scholar
- Rosenthal E, Marty P: Actualités sur la leishmaniose viscérale méditerranéenne. Update on visceral Mediterranean leishmaniasis. La Revue de médecine interne. 2009, 30: : S24-S28.View ArticleGoogle Scholar
- Marty P, Pomares-Estran C, Hasseine L, Delaunay P, Haas H, Rosenthal E: Actualités sur les leishmanioses en France. Leishmaniases in France: an update. Archives de Pediatrie. 2009, 16: S96-S100.PubMedView ArticleGoogle Scholar
- Machado de Assis TS, Basques Caligiornea R, Sierra Romerob GA, Rabelloa A: Detection of Leishmania kDNA in human serum samples for the diagnosis of visceral leishmaniasis. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2009, 103: 1269-1272. 10.1016/j.trstmh.2008.07.013.View ArticleGoogle Scholar
- Moltóa A, Mateoa L, Lloverasb N, et al: Visceral leishmaniasis and macrophagic activation syndrome in a patient with rheumatoid arthritis under treatment with adalimumab. Joint Bone Spine May. 2010, 77: 271-273. 10.1016/j.jbspin.2010.01.011.View ArticleGoogle Scholar
- Quilici M, Dunan S, Mary C: La leishmaniose viscerale méditerranéenne dans le sud-est de la France. Sem Hop Paris. 1989, 65: 2155-2161.Google Scholar
- Cascio A, Gradoni L, Scarlata F, et al: Epidemiologic surveillance of visceral leishmaniasis in Sicily, Italy. Am J Trop Med Hyg. 1997, 57: 75-78.PubMedGoogle Scholar
- Sellami H, Ayadi A, Makni F: La leishmaniose viscérale de l'adulte au cours d'une immunode 'pression non liée au SIDA. Bull Soc Fr Parasitol. 1998, 16: 80-85.Google Scholar
- Hernandez-Perez J, Yebra-Bango M, Jimenez-Martinez E, et al: Visceral leishmaniasis (Kala-azar) in solid organ transplantation: report of five cases and review. Clin Infect Dis. 1999, 29: 918-921. 10.1086/520457.PubMedView ArticleGoogle Scholar
- Aoun K, Kaaroud H, Hamzaoui S, et al: Particularités de la leishmaniose viscérale de l'adulte non infecté par le VIH en tunisie. Med Trop. 2004, 64: 160-162.Google Scholar
- Afrit M, Azzabi S, Cherif E, et al: Modes de révélation de la leishmaniose viscérale dans un service de médecine interne. La Revue de médecine interne. 2008, 29 (Suppl 1): S102-View ArticleGoogle Scholar
- Hakem D, Médaoud S, Boucelma M, et al: Leishmanioses viscérales: particularités cliniques et difficultés thérapeutiques. La Revue de médecine interne. 2007, 28 (Suppl 1): 102-103.View ArticleGoogle Scholar
- Djidingar D, Chippaux JP, Gragnic G, et al: La leishmaniose viscérale au Niger: six nouveaux cas confirmés parasitologiquement. « Clinique ». 1997, Manuscrit n° 1754Google Scholar
- Pagliano P, Carannante N, Rossi M, Gramiccia M, Gradoni L, Faella FS, Gaeta GB: Visceral leishmaniasis in pregnancy: a case series and systematic review of literature. J Antimicrob Chemother. 2005, 55 (Suppl 2): 229-233.PubMedView ArticleGoogle Scholar
- Aoun K, Kooli C, Bouratbine A, et al: Aspects épidémiologiques et cliniques de la leishmaniose viscérale de l'adulte en Tunisie. Méd Mal Infect. 2002, 32: 387-392. 10.1016/S0399-077X(02)00386-4.View ArticleGoogle Scholar
- Desjeux P: The increase in risk factors for leishmaniasis worldwide. Trans R Soc Trop Med Hyg. 2001, 95: 239-43. 10.1016/S0035-9203(01)90223-8.PubMedView ArticleGoogle Scholar
- Lakhdar Idrissi M, El Ouardi M, Atmani S, et al: La leishmaniose viscérale infantile: à propos de 209 cas Infantile visceral leishmaniasis: about 209 cases. Journal de pédiatrie et de puériculture. 2007, 20: 136-141.View ArticleGoogle Scholar
- Benlbarhdadi I, Ajana FZ, Benazouz M, et al: La leishmaniose viscerale chez l'adulte. A propos de 6 cas. Médecine du Maghreb. 1995, n° 50Google Scholar
- Pagliano P, Rossi1 M, Rescigno C, Altieri S, Coppola MG, Gramiccia M, Scalone A, Gradoni L, Faella F: Mediterranean visceral leishmaniasis in HIV-negative adults: a retrospective analysis of 64 consecutive cases (1995-2001). Journal of Antimicrobial Chemotherapy. 2003, 264-268. 52
- Dedet JP: Leishmanies, leishmanioses. Biologie, clinique et thérapeutique. Encycl Méd Chir, Maladies infectieuses. 2001, 11-8-506-A-10Google Scholar
- Dedet P: Les leishmanioses. Encycl. Méd. Chir. Maladies infectieuses. 1986, 8-8094 A10, 4Google Scholar
- Izri A, Belazzoug S: Diagnostic de laboratoire des leishmanioses rencontrées en Algérie. Revue francophone des laboratoires, Novembre. 2007, 396 (suppl):
- Dedet JP: Les leishmanioses Ellipses. Edited by: Aupelf/Uref, Paris. 1999, 253-Google Scholar
- Sundar S, Singh RK, Maurya R, et al: Serological diagnosis of Indian visceral leishmaniasis: direct agglutination test versus rK39 strip test. Trans R Soc Trop Med Hyg. 2006, 100: 533-7. 10.1016/j.trstmh.2005.08.018.PubMedView ArticleGoogle Scholar
- Dana M: La leishmaniose viscérale méditerranéenne et son diagnostic immunologique. La Nouvelle Presse Médicale. 1972, 20: 1363-Google Scholar
- Rolland L, Monjour L, Danis M, Gentilini M: Leishmaniose viscérale. Diagnostic par immuno-empreinte. La Presse Médicale. 1992, 21: 971-973.PubMedGoogle Scholar
- Abdelmoula MS, Mhamdi Z, Amri F, et al: La leishmaniose viscérale chez l'enfant. Maghreb Medical. 2003, 23: 40-42.Google Scholar
- Dedet JP: Les leishmanioses: actualités. Press Med. 2000, 29: 1019-1026.Google Scholar
- Pearson RD, De Queirozsouza A: Clinical spectrum of leishmaniasis. Clin Infect Dis. 1995, 22: 1-13.View ArticleGoogle Scholar
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