Figure 4

CARS and PYROXD1 genes were identified by Pearson correlation, Spearman rank correlation, and Linear Models analyses. (A) Overlapping genes identified by the Spearman rank correlation, Pearson correlation and Linear Models microarray analyses are indicated by the venn diagram. Two PYROXD1 probesets (denoted as PYROXD1 _A and PYROXD1 _B ) and CARS (probeset ID 212971_at) were identified by all three analyses. (B) The box and whisker plot examines and compares normalized mRNA expression of CARS and PYROXD1 among breast tumors scored as 0, 0/1+, 1+, 1+/2+, 2+ or 3+ for cell surface NIS protein. As shown by the scatter plot, CARS and PYROXD1 mRNA levels correlate with cell surface NIS protein levels. The length of the box represents the interquartile range (i.e., the middle 50% of the data). The median (line through the middle of each box), the lower quartile (bottom line of each box), and the upper quartile (top line of each box) are also specified on the plot for each level of cell surface NIS protein. The sample minimum and maximum values are represented as T-shaped lines extending from the ends of the box. Maximum outliers (gray squares) and minimum outliers (black diamonds) are also plotted.