Figure 5From: Transformation of Epichloë typhina by electroporation of conidiaSouthern blot analysis of transformed Epichloë typhina lines. Genomic DNA was digested with EcoRI and probed with a portion of the GFP gene (608 bp amplicon between nucleotide 64 and 671 of the GFP open reading frame) that was labelled with digoxigenin. The presence of a single band in most lanes indicates a single insertion event in those transformed lines. The different size of the bands indicates unique transformation events.Back to article page