Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)

Table 2 Summary of the experimental conditions comprising the genotypes, tissues, treatments and the number of biological and technical replicates used in the present study.

Genotype	Tissue/Treatment	No. of biological replicate	No. of technical replicates per biological replicate	Whole datasets	Different tissues	Stressed leaves
94/1-30	leave	3	6	+	+	-
	floral bud	3	6	+	+	-
	root	3	6	+	+	-
94/1-97	leave	3	6	+	+	-
	floral bud	3	6	+	+	-
	root	3	6	+	+	-
93/1-119	leave	3	6	+	+	+
	floral bud	3	6	+	+	-
	root	3	6	+	+	-
	wounded leave	3	6	+	-	+
	heat stressed leave	3	6	+	-	+
	leave inoculated with black spot	3	6	+	-	+

For each tissue or treatment three biological replicates were tested with six technical replicates. The technical replicates comprised of three independent qPCR runs in which each amplification reaction was repeated once, respectively. The pluses (+) sum up the samples of the corresponding datasets of the stability analysis.

ISSN: 1756-0500