From: A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation
Primer sequences (CpG sites in bold)
Annealing temperature
(°C)
Amplicon size (bp)
Number of CpGs between primers
Primer Set
F: 5'-GCG AGGTATTGTTTTATTTGGGA-3'
R: 5'-CG CCG TTTCTTAAACC-3'
54°C
141
8