Figure 3From: Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real-time PCRThe pairwise variation (V) of the candidate reference genes for accurate normalization in the two tissue samples in the different conditions: after blood feeding, T. rangeli infection or T. cruzi infection. The threshold value (0.15) is marked with a dashed line and indicates that the use of only the two most stable genes was sufficient to obtain an accurate normalization in all of the analyzed groups (V 2/3 < 0.15). SG, salivary gland.Back to article page