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Table 3 Ranking of reference genes using ΔCq and GeNorm

From: Microarray-based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress

 

ΔCq

GE Norm

   

M values of Ref genes

Ref. Genes

Sum of Ref Gene values

Ranking

Greenhouse

Field

All samples

act

40

2

1.834

0.503

1.88

L2

137

5

3.604

0.735

3.55

VH1-IK

408

7

1.974

0.496

1.884

aladin-related

1243

8

2.166

0.603

2.072

TIF

200

6

1.921

0.552

1.829

TIF-GTP

110

4

1.89

0.594

1.837

PADCP

39

1

2.092

0.674

2.007

ubiq

54

3

1.885

0.58

1.817

  1. Ranking of reference genes using two different methods, ΔCq and the software GeNorm (http://medgen.ugent.be/~jvdesomp/genorm/). The normalisation with ΔCq was performed with all samples while with GeNorm it was performed with all samples, with greenhouse samples and with field samples individually.
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BMC Research Notes

ISSN: 1756-0500

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