Figure 1
Comparison of normalization strategies on expression of two common osteogenesis markers. Relative quantification of OC and ALP expression during suture fusion in Fgfr2cC342Y/+ mice is dependent on which reference genes are used. Target gene expression was normalized to the geometric mean of Cyc1 - Gapdh - Canx (a and d), the single most stable gene - Canx (b and e) or the least stable gene – 18 S rRNA (c and f). Relative gene expression in wildtype sutures, normalized to the different reference genes, is also presented (g and h). Expression is displayed as fold change relative to day 0 wildtype. N = 6. * Represents a significant difference between two groups ( p < 0.05). # Represents a significant difference to Day 0 WT value (p < 0.05).