Figure 2

Comparison of total RNA and Ribo-Zero-subtracted RNA from Drosophila ananassae. The reads from both samples were mapped with BWA against the D. ananassae scaffolds. Each scaffold was then computationally divided into 100 kbp fragments and the number of reads mapping to the fragment were counted. When scaffolds were <100 kbp, the entire scaffold was counted. The last fragment of each scaffold was always <100 kbp. Fragments containing annotated rRNA are shifted right of the diagonal (gray line) due to decreased representation in the Ribo-Zero-treated RNA. Meanwhile, fragments without rRNA are shifted leftward of the diagonal (gray line) because of their increased abundance relative to the entire sequenced population in the subtracted samples.