Oligo name | Oligo sequence | Orientation | Primer set | Substrate to PCR | Initial denatur alization | Amplification program | Cycles | Final elongation | Amplicon size | ||
---|---|---|---|---|---|---|---|---|---|---|---|
Denaturalization | Alignment | Elongation | |||||||||
OLIGO-rnaF | CTTGGCGCTAAGCTGCCGC | Forware | AMPLICON -mRNA | mRNA | 94°/3.5 min | 94°/30 seg | 60°/45 seg | 72°/30 seg | 42 | 72°/15 min | 726 pb |
OLIGO-rnaR | CCTGCTCTGGAGTCTTGGTG | Reverse | |||||||||
OLIGO-gene1F | AGCTGGCACTCCCCAGAAT | Forware | OLIGO-gene1 | gDNA | 94°/5 min | 94°/1 min | 60°/45 seg | 72°/1 min | 35 | 72°/10 min | 946 pb |
OLIGO-gene1R | GGCTGAGGCACAGAGAAACT | Reverse | |||||||||
OLIGO-gene2F | CTCCCTACCAAAGTGGCTAG | Forware | OLIGO-gene2 | gDNA | 94°/5 min | 94°/1 min | 60°/45 seg | 72°/35 seg | 30 | 72°/10 min | 769 pb |
OLIGO-gene2R | TGTGGAAAGTTGGGGTTTTC | Reverse | |||||||||
OLIGO-gene3F | GAAAACCCCAACTTTCCACA | Forware | OLIGO-gene3 | gDNA | 94°/5 min | 94°/1 min | 60°/45 seg | 72°/30 seg | 30 | 72°/10 min | 723 pb |
OLIGO-gene3R | CCAGGCGAGCAGTCTGTAAC | Reverse | |||||||||
OLIGO-gene4F | CCTCTCCTGCAACCAATCAG | Forware | OLIGO-gene4 | gDNA | 94°/5 min | 94°/1 min | 60°/45 seg | 72°/45 seg | 30 | 72°/10 min | 807 pb |
OLIGO-gene4R | CCCGAATATCCTGGGCTCT | Reverse | |||||||||
OLIGO-gene5F | CCGGAAGAGCCCAGGATA | Forware | OLIGO-gene5 | 94°/5 min | 94°/1 min | 60°/45 seg | 72°/1 min | gDNA | 35 | 72°/10 min | 967 pb |
OLIGO-gene5R | CCGTTTTGGCAGGAATACAT | Reverse |