Characterization of genes involved in ceramide metabolism in the Pacific oyster (Crassostrea gigas)

BMC Research Notes

Table 2 Top BLASTn and BLASTx hits for the Crassostrea gigas genes Cg-sptlc1 , Cg-3KDSR , Cg-GlcCer , Cg-AC

Gene	Top BLASTn Hit	BLASTn Similarity	2^ndBLASTn Hit	2^ndBLASTn Similarity	Top BLASTx Hit
Cg-sptlc1	Xenopus tropicalis (NM_001079574)	71%	Saccoglossus kowalevskii (SM_002730516)	70%	Pongo abelii (Q5R9T5)
Cg-3KDSR	Rattus norvegicus (NM_001108342)	68%	Saccoglossus kowalevskii (SM_002740331)	76%	Mus musculus (Q6GV12)
Cg-GlcCer	Pediculus humanus corporis (SM_002431306)	66%	Xenopus laevis (NM_001090475)	66%	Xenopus tropicalis (Q5BL38)
Cg-AC	Sebastes schlegelii (AB491143)	67%	-	-	Rattus norvegicus (Q6P71)

The BLAST algorithm was used to find the best matches in the NCBI nucleotide database (BLASTn) and UniProt/SwissProt protein database (BLASTx). For each gene, the top two BLASTn matches are given along with the percent sequence similarity. Sequence identity values shown represent local similarity as reported by BLAST. Cg-AC only had one top BLASTn hit. The top BLASTx protein hit is also given. GenBank Accession Numbers are in parentheses next to the species name of the top hit.

ISSN: 1756-0500