Mitochondrial fission and fusion in Dictyostelium discoideum: a search for proteins involved in membrane dynamics
© Schimmel et al.; licensee BioMed Central Ltd. 2012
Received: 22 July 2012
Accepted: 6 September 2012
Published: 14 September 2012
Mitochondrial morphology is maintained by two distinct membrane events -fission and fusion. Altering these conserved processes can disrupt mitochondrial morphology and distribution, thereby disrupting the organelle’s functionality and impeding cellular function. In higher eukaryotes, these processes are mediated by a family of dynamin-related proteins (DRP’s). In the lower eukaryotes, for instance Dictyostelium discoideum, mitochondrial fission and fusion have been implicated but not yet established. To understand the overall mechanism of these dynamics across organisms, we developed an assay to identify fission and fusion events in Dictyostelium and to assess the involvement of the mitochondrial proteins, MidA, CluA, and two DRP’s, DymA and DymB.
Using laser scanning confocal microscopy we show, for the first time, that lower eukaryotes mediate mitochondrial fission and fusion. In Dictyostelium, these processes are balanced, occurring approximately 1 event/minute. Quantification of the rates in midA-, cluA-, dymA - , or dymB - strains established that MidA appears to play an indirect role in the regulation of fission and fusion, while the DRP’s are not essential for these processes. Rates of fission and fusion were significantly reduced in cluA-cells, indicating that CluA is necessary for maintaining both fission and fusion.
We have successfully demonstrated that Dictyostelium mitochondria undergo the dynamic processes of fission and fusion. The classical mediators of membrane dynamics - the DRP’s – are not necessary for these dynamics, whereas CluA is necessary for both processes. This work contributes to our overall understanding of mitochondrial dynamics and ultimately will provide additional insight into mitochondrial disease.
KeywordsMitochondria Fission Fusion Mitochondrial morphology Dictyostelium discoideum MidA CluA Dynamin
Mitochondria are cellular organelles that produce energy in the form of ATP, play a central role in cellular metabolic pathways, and are essential for regulating apoptosis [1–3]. In most organisms, mitochondria are reticular, highly branched, complex structures . The maintenance of this structure is vital for the success of these and other essential cellular functions. In both yeast and mammalian cells, this tubular structure is maintained by a balance of two dynamic and highly conserved processes: fission and fusion. Fission is the process by which mitochondria divide, while fusion occurs when neighbouring organelles join together to become one [1, 4, 5].
Disrupting these processes alters not only the mitochondrial morphology but also the distribution of mitochondria throughout the cell. Uneven distribution decreases the targeting efficiency of critical metabolites, such as ATP . It has also been suggested that fission and fusion ensure a healthy population of mitochondria and protect against potential damage or loss of the mitochondrial DNA which contains the genes necessary to carry out cellular respiration . These processes play a significant role in the regulation of apoptosis [2, 8–10] and have been implicated in normal developmental pathways as well as in the progression of neurodegenerative diseases such as Charcot-Marie Tooth or dominant optic atrophy [2, 11–13].
To understand the mechanism of these mitochondrial processes, studies have been undertaken in yeast and mammalian cells - the results of which indicate that mitochondrial fission and fusion are mediated by a family of dynamin-related proteins (DRP’s) [1, 14–17]. DRP’s function in membrane remodelling events such as budding, organelle fission and fusion, endocytosis, and cytokinesis [18–21]. These proteins do not work alone; in mitochondria, multi-component machines—fission and fusion complexes—are responsible for remodelling the inner and outer mitochondrial membranes to allow a tubule to divide or fuse [5, 17]. Although the processes themselves are highly conserved, variations in the protein composition of the complexes do occur. For example, mammalian cells lack the adaptors Mdv1 and Ugo1 that function in yeast cells [1, 3, 17, 22].
In an effort to understand the overall mechanism of these processes and the differences between organisms, a study of the lower eukaryote, Dictyostelium discoideum, was undertaken. It has been suggested that Dictyostelium cells do carry out mitochondrial fission [23, 24], but no actual fission or fusion events have been identified. Thus, we developed an in vivo assay to determine if Dictyostelium cells carry out the processes of mitochondrial fission and fusion and to identify potential molecular players in these pathways. Dictyostelium expresses a number of proteins that are associated (based on function, homology, or localization) with the mitochondria, including two DRP and three dynamin-like proteins . For this study, we chose the two DRP’s, DymA and DymB, and two non-DRP’s, MidA and CluA. MidA was chosen because it does not induce a change in mitochondrial morphology yet plays a significant role in mitochondrial function . CluA was chosen because deletion of this protein induces a clustered mitochondrial morphology comparable to a fission defect in yeast cells [26, 27]. Here, we present the first time-lapse images showing actual fission and fusion events in Dictyostelium cells. We quantified these events to establish that mitochondrial fission and fusion are balanced processes in these cells. In addition, we have begun to determine which proteins are essential for these processes. Our results reveal that MidA and the DRP’s - DymA and DymB - are not required for mitochondrial fission or fusion. Interestingly, CluA appears to have some role in both processes as demonstrated by a decrease in both rates in the protein’s absence. The results from this work will further our knowledge of the mechanisms of fission and fusion, ultimately contributing to a better understanding of both membrane dynamics and, more importantly, mitochondrial disease.
Strain culture and growth conditions
All Dictyostelium discoideum strains described here were obtained from Dicty Stock Center , AX4 (wild-type) was deposited by Bill Loomis, cluA - by Margaret Clarke, dymA - and dymB - by Dietmar Manstein, and midA - by Ricardo Escalante. The strains were cultured axenically in liquid HL5 medium supplemented with streptomycin (Fisher) (final concentration 300 μg/ml) and ampicillin (Fisher) (final concentration 150 μg/ml) at 22 °C shaking at 125 rpm .
Confocal microscopy of Dictyostelium mitochondria
AX4, midA - , and cluA - cells were diluted to 3 X 104 cells/ml in Lo-Flo liquid media (Formedium) until cells reached log phase (4–7 days). dymA- and dymB- cells were diluted into HL-5 instead of Lo-Flo. Log phase cells (5 ml) were washed by centrifuging at 500 x g for 4 minutes and resuspended in 5 ml of Lo-Flo (including dymA- and dymB - ) plus 1 μl of 1 mM MitoTracker Red CMXRos (Invitrogen) . Cells were incubated at room temperature with shaking for 3–5 hours, washed twice with Lo-Flo, and resuspended in 5 ml Lo-Flo media. Washed and stained cells (0.5 ml) were placed in Lab TekII 4-well chambered coverglass (Nalge Nunc International) for imaging.
Quantification of mitochondrial fission and fusion
Quantified cells were imaged on a Zeiss laser scanning LSM Pascal confocal microscope using a pinhole of 144 (1.36 airy units), resulting in an optical slice of 1.1 μm. A single plane was imaged every 677.38 milliseconds for 100 seconds or until bleaching occurred. Fission and fusion events were identified in a minimum of 35 cells for each strain. Fission was identified when one organelle became two if, prior to this, no out-of-focus mitochondria had been visible. Fusion was identified when two mitochondria rotated around one another for a couple of frames (They never resolved into one during this time.) then became one. If two organelles came together, or split apart and were in their original state by the next frame, this was classified as a “drive by” and not quantified. Rates were calculated by averaging the number of events/min/cell for each strain and are presented as mean ± standard error. Statistical analysis was performed using JMP 9.0.0 (SAS Institute, Inc) software. Wilcoxon rank-sum nonparametric t-tests were conducted for statistical comparisons of fission and fusion within and among strains; p-values less than 0.05 were considered statistically significant.
To be as stringent as possible with this assay, the data presented here has been repeated independently by a second individual who obtained statistically similar results (data not shown).
In an effort to confirm that the identified fission and fusion events are not from mitochondria entering and leaving the focal plane, small z-stacks were imaged. These included 3 planes of 1.1 μm depth, images were flattened and quantified as above.
Dictyostelium mitochondria undergo fission and fusion
MidA is not involved in mitochondrial fission or fusion
There are numerous proteins, based on function or homology, which may play a role in mitochondrial fission or fusion. We chose four of these proteins and used our assay to determine if they were involved in these processes. MidA is a putative methyltransferase that is targeted to the mitochondria and is required for mitochondrial complex I function ; it has been identified in Dictyostelium and humans  with a possible homolog in budding yeast . Mitochondrial morphology of midA- cells is not altered from wild-type ; thus, we did not expect MidA to be involved in fission or fusion. Interestingly, when the processes were quantified in midA- cells, the fission rate (0.59 ± 0.15 events/min) was significantly decreased as compared to the wild-type (p-value = 0.0056). In contrast, the average rate of fusion (0.78 ± 0.18 events/min) was not statistically different from wild-type (p-value = 0.17). Despite the decrease of fission, the rates between the two processes are balanced (p-value = 0.527) and there is no resulting morphological change so, most likely, MidA is not directly involved in mitochondrial fission (Figure 3, Additional file 2).
CluA is necessary for maintaining rates of fission and fusion
Classical DRP’s are not essential for mitochondrial dynamics in Dictyostelium
DymB transiently localizes to the mitochondria - perhaps to complete post-translational processing- although deletions of DymB do not alter mitochondrial morphology . In an effort to determine if DymB functions in mitochondrial dynamics, dymB - mitochondria were examined and the rates of fission and fusion were found to be balanced (p-value = 0.83) and occur at a rate of 0.88 ± 0.14 fission events/min and 0.92 ± 0.14 fusion events/min (Additional file 5). These rates are not significantly different from AX4 (fission: p-value = 0.38; fusion: p-value = 0.99), therefore DymB is also not required for mitochondrial fission or fusion.
Here, we presented our findings on the processes of fission and fusion in Dictyostelium cells. Using our in vivo quantification assay, we demonstrated for the first time that, although Dictyostelium mitochondria are spherical in structure, their mitochondria do undergo fission and fusion. In further support of this result, Dictyostelium mitochondria morphology is very similar in appearance to peroxisomes. Peroxisomes have been shown to undergo the dynamic process of fission (though not fusion) ; thus, even spherical organelles require some dynamics. Based on the spherical structure, one might expect fission to occur more often than fusion to create the spheres rather than tubules that are found in other organisms. In Dictyostelium, the events are balanced, occurring approximately 1 event/min; interestingly, this is about twice as often as the rates published for yeast .
In an effort to begin identifying potential players in the fission and fusion processes, we examined four proteins that have some known association with the mitochondria. MidA appears to significantly alter mitochondrial function by disrupting the assembly of complex I in the electron transport chain . Mutations in this protein result in numerous deleterious phenotypes ranging from small cell size and slow growth rate to a decrease in phagocytosis and macropinocytosis as well as a 70% reduction in ATP production .
Although fission and fusion are slightly decreased (fission more so than fusion), the rates are still balanced and mitochondrial morphology is not altered. This decrease in mitochondrial dynamics may be an indirect result of the inadequately functioning mitochondria. The work presented here suggests that MidA does not play a direct role in the processes of fission and fusion.
Transmission electron microscope images of cluA - cells indicate that the mitochondria are connected to one another, forming the characteristic cluster . Our results demonstrate that there is a significant decrease in both fission and fusion processes of these mitochondria, resulting in a fission/fusion event approximately every two minutes. These collective results suggest that perhaps in the absence of CluA fission and/or fusion may be initiated but not completed, resulting in a connected cluster of organelles. The inability to complete the initiated events may be due to a loss of interaction with the cytoskeleton. CluA has homology to the protein-protein interaction region of the kinesin light chain - the tetratricopeptide repeats (TPR) domain  - suggesting that CluA could interact with kinesin and thus, the cytoskeleton. Studies performed in flies and plants support this conclusion [33, 34]. However, there are many TPR proteins that do not interact with kinesin; an example is Fis1, a TPR protein which is required for mitochondrial fission in yeast and mammalian cells . Ultimately, to tease out the specific role CluA plays in these processes, further studies will need to be pursued; for example, identifying interacting partners of CluA will help to determine if it connects to the cytoskeleton and/or to other potential fission and fusion proteins.
Finally, we looked at the classical proteins that mediate membrane remodelling events, the DRP’s. Previous work suggests that both DymA and DymB have primary functions elsewhere in the Dictyostelium cell - endosomal pathway and vacuole/peroxisome dynamics respectively [20, 21]. However, several proteins have been co-opted to work in multiple organelle remodeling events. In yeast for instance, both the peroxisome and the mitochondria use the DRP, Dnm1, to carry out fission . Thus, we analyzed both DymA and DymB to see if one of these proteins may have been co-opted to work in multiple remodelling events. dymA - and dymB - mitochondria undergo fission and fusion about once every minute (equivalent to the rate found in wild-type cells) thus, DymA and DymB are not essential for these processes and have not been co-opted to mediate multiple organelle remodelling events. Although DRP’s have been found to be responsible for mediating fission and fusion in all organisms studied to date, this is the first time it has been demonstrated that the classical DRP’s are not required for mitochondrial dynamics. It is possible that the DRP-like proteins play this role in Dictyostelium or more likely the FtsZ homologs, FszA and FszB, as suggested by Gilson et al., 2003 .
In summary, we have shown for the first time that Dictyostelium mitochondria undergo fission and fusion like all organisms studied to date. The rates of these events are balanced, occurring at a rate of approximately 1 event/min. MidA is not essential for these processes nor are the classical DRP’s, DymA and DymB, while CluA is necessary for both fission and fusion. This work contributes to our understanding of the overall mechanism of mitochondrial fission and fusion, but it is lacking the identification of the proteins specifically involved in these processes. Our goal is to use this assay to continue the search for these proteins, specifically asking if FszA or FszB might be responsible for mediating mitochondrial fission or fusion in place of the DRP’s.
Availability of supporting data
The data sets supporting the results of this article are included within the article and its additional files.
Thank you to Brittany Sexson who established the Dictyostelium model system in our lab and to LaRhonda Apata and Jordan Bird who preliminarily established that fission and fusion are balanced. Thank you to Margaret Clarke, Vladimir Lupashin and Bhupinder Vohra for critiques of this manuscript. The project described was supported by grants from the National Center for Research Resources (5P20RR016460-11) and the National Institute of General Medical Sciences (8 P20 GM103429-11) from the National Institutes of Health. The Zeiss LSM Pascal confocal microscope used for image acquisition was funded by NSF Award Number 0215847.
- Osteryoung KW, Nunnari J: The division of endosymbiotic organelles. Science. 2003, 302 (5651): 1698-1704. 10.1126/science.1082192.PubMedView ArticleGoogle Scholar
- Karbowski M, Youle RJ: Dynamics of mitochondrial morphology in healthy cells and during apoptosis. Cell Death Differ. 2003, 10 (8): 870-880. 10.1038/sj.cdd.4401260.PubMedView ArticleGoogle Scholar
- Scott I, Youle RJ: Mitochondrial fission and fusion. Essays Biochem. 2010, 47: 85-98. 10.1042/bse0470085.PubMedView ArticleGoogle Scholar
- Johnson LV, Walsh ML, Chen LB: Localization of mitochondria in living cells with rhodamine 123. Proceedings of the National Academy of Science, USA. 1980, 77: 990-994. 10.1073/pnas.77.2.990.View ArticleGoogle Scholar
- Nunnari J, Marshall W, Straight A, Murray A, Sedat JW, Walter P: Mitochondrial transmission during mating in S. cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mtDNA. Molecular Biology of the Cell. 1997, 8 (7): 1233-1242.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen H, Chan DC: Mitochondrial dynamics–fusion, fission, movement, and mitophagy–in neurodegenerative diseases. Human Molecular Genetics. 2009, 18 (R2): R169-R176. 10.1093/hmg/ddp326.PubMedPubMed CentralView ArticleGoogle Scholar
- Detmer SA, Chan DC: Functions and dysfunctions of mitochondrial dynamics. Nat Rev Mol Cell Biol. 2007, 8 (11): 870-879. 10.1038/nrm2275.PubMedView ArticleGoogle Scholar
- Karbowski M, Lee YJ, Gaume B, Jeong SY, Frank S, Nechushtan A, Santel A, Fuller M, Smith CL, Youle RJ: Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis. J Cell Biol. 2002, 159 (6): 931-938. 10.1083/jcb.200209124.PubMedPubMed CentralView ArticleGoogle Scholar
- Frank S, Gaume B, Bergmann-Leitner ES, Leitner W, Robert EG, Catez F, Smith CL, Youle RJ: The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis. Developmental Cell. 2001, 1: 515-525. 10.1016/S1534-5807(01)00055-7.PubMedView ArticleGoogle Scholar
- Jin C, Reed JC: Yeast and apoptosis. Nat Rev Mol Cell Biol. 2002, 3 (6): 453-459. 10.1038/nrm832.PubMedView ArticleGoogle Scholar
- Zuchner S, Mersiyanova IV, Muglia M, Bissar-Tadmouri N, Rochelle J, Dadali EL, Zappia M, Nelis E, Patitucci A, Senderek J, Parman Y, Evgrafov O, Jonghe PD, Takahashi Y, Tsuji S, Pericak-Vance MA, Quattrone A, Battologlu E, Polyakov AV, Timmerman V, Schroder JM, Vance JM: Mutations in the mitochondrial GTPase mitofusin 2 cause Charcot-Marie-Tooth neuropathy type 2A. Nat Genet. 2004, 36 (5): 449-451. 10.1038/ng1341. Epub 2004 Apr 2004PubMedView ArticleGoogle Scholar
- Delettre C, Lenaers G, Griffoin J-M, Gigarel N, Lorenzo C, Belenguer P, Pelloquin L, Grosgeorge J, Turc-Carel C, Perret E, Astarie-Dequeker C, Lasquellec L, Arnaud B, Ducommun B, Kaplan J, Hamel CP: Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy. Nature Genetics. 2000, 26 (2): 207-210. 10.1038/79936.PubMedView ArticleGoogle Scholar
- Alexander C, Votruba M, Pesch UEA, Thiselton DL, Mayer S, Moore A, Rodriguez M, Kellner U, Leo-Kottler B, Auburger G, Bhattacharya SS, Wissinger B: OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28. Nature Genetics. 2000, 26 (2): 211-215. 10.1038/79944.PubMedView ArticleGoogle Scholar
- Otsuga D, Keegan BR, Brisch E, Thantcher JW, Hermann GJ, Bleazard W, Shaw J: The dynamin GTPase, Dnm1p, controls mitochondrial morphology in yeast. Journal of Cell Biology. 1998, 143: 333-349. 10.1083/jcb.143.2.333.PubMedPubMed CentralView ArticleGoogle Scholar
- Bleazard W, McCaffery JM, King EJ, Bale S, Mozdy A, Tieu Q, Nunnari J, Shaw JM: The dynamin-related GTPases, Dnm1, regulates mitochondrial fission in yeast. Nature Cell Biology. 1999, 1: 298-304. 10.1038/13014.PubMedPubMed CentralView ArticleGoogle Scholar
- Sesaki H, Jensen RE: Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape. Journal of Cell Biology. 1999, 147 (4): 699-706. 10.1083/jcb.147.4.699.PubMedPubMed CentralView ArticleGoogle Scholar
- Shaw JM, Nunnari J: Mitochondrial dynamics and division in budding yeast. Trends in Cell Biology. 2002, 12 (4): 178-184. 10.1016/S0962-8924(01)02246-2.PubMedPubMed CentralView ArticleGoogle Scholar
- Osteryoung KW: Organelle fission in eukaryotes. Curr Opin Microbiol. 2001, 4 (6): 639-646. 10.1016/S1369-5274(01)00263-6.PubMedView ArticleGoogle Scholar
- Sever S: Dynamin and endocytosis. Curr Opin Cell Biol. 2002, 14 (4): 463-467. 10.1016/S0955-0674(02)00347-2.PubMedView ArticleGoogle Scholar
- Wienke DC, Knetsch MLW, Neuhaus EM, Reedy MC, Manstein DJ: Disruption of a Dynamin Homologue Affects Endocytosis, Organelle Morphology, and Cytokinesis in Dictyostelium discoideum. Mol Biol Cell. 1999, 10 (1): 225-243.PubMedPubMed CentralView ArticleGoogle Scholar
- Rai A, Nothe H, Tzvetkov N, Korenbaum E, Manstein D: Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles. Cellular and Molecular Life Sciences. 2011, 68: 2751-2767. 10.1007/s00018-010-0590-5.PubMedPubMed CentralView ArticleGoogle Scholar
- Scott SV, Cassidy-Stone A, Meeusen SL, Nunnari J: Staying in aerobic shape: how the structural integrity of mitochondria and mitochondrial DNA is maintained. Curr Opin Cell Biol. 2003, 15 (4): 482-488. 10.1016/S0955-0674(03)00070-X.PubMedView ArticleGoogle Scholar
- Gilson PR, Yu X-C, Hereld D, Barth C, Savage A, Kiefel BR, Lay S, Fisher PR, Margolin W, Beech PL: Two Dictyostelium Orthologs of the Prokaryotic Cell Division Protein FtsZ Localize to Mitochondria and Are Required for the Maintenance of Normal Mitochondrial Morphology. Eukaryotic Cell. 2003, 2 (6): 1315-1326. 10.1128/EC.2.6.1315-1326.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Fields SD AQ, Heuser J, Clarke M: Mitochondrial membrane dynamics are altered in cluA- mutants of Dictyostelium. Journal of Muscle Research and Cell Motility. 2002, 23: 829-838. 10.1023/A:1024492031696.PubMedView ArticleGoogle Scholar
- Torija P, Vicente JJ, Rodrigues TB, Robles A, Cerdan S, Sastre L, Calvo RM, Escalante R: Functional genomics in Dictyostelium: MidA, a new conserved protein, is required for mitochondrial function and development. Journal of Cell Science. 2006, 119 (6): 1154-1164. 10.1242/jcs.02819.PubMedView ArticleGoogle Scholar
- Fields SD, Conrad MN, Clarke M: The S. cerevisiae CLU1 and D. discoideum cluA genes are functional homologues that influence mitochondrial morphology and distribution. Journal of Cell Science. 1998, 111 (12): 1717-1727.PubMedGoogle Scholar
- Zhu Q, Hulen D, Liu T, Clarke M: The cluA- mutant of Dictyostelium identifies a novel class of proteins required for dispersion of mitochondria. Proceedings of the National Academy of Sciences. 1997, 94 (14): 7308-7313. 10.1073/pnas.94.14.7308.View ArticleGoogle Scholar
- Fey P, Kowal AS, Gaudet P, Pilcher KE, Chisholm RL: Protocols for growth and development of Dictyostelium discoideum. 2007, 2 (6): 1307-1316.Google Scholar
- Poot M, Zhang YZ, Krämer JA, Wells KS, Jones LJ, Hanzel DK, Lugade AG, Singer VL, Haugland RP: Analysis of mitochondrial morphology and function with novel fixable fluorescent stains. Journal of Histochemistry & Cytochemistry. 1996, 44 (12): 1363-1372. 10.1177/44.12.8985128.View ArticleGoogle Scholar
- Carilla-Latorre S, Gallardo ME, Annesley SJ, Calvo-Garrido J, Grana O, Accari SL, Smith PK, Valencia A, Garesse R, Fisher PR, Escalante R: MidA is a putative methyltransferase that is required for mitochondrial complex I function. Journal of Cell Science. 2010, 123 (10): 1674-1683. 10.1242/jcs.066076.PubMedView ArticleGoogle Scholar
- UniProt CT: Reorganizing the protein space at the Universal Protein Resource (UniProt). Nucleic Acids Res. 2012, 40: D71-D75.View ArticleGoogle Scholar
- Logan DC, Scott I, Tobin AK: The genetic control of plant mitochondrial morphology and dynamics. The Plant Journal. 2003, 36 (4): 500-509. 10.1046/j.1365-313X.2003.01894.x.PubMedView ArticleGoogle Scholar
- Cox RT, Spradling AC: clueless, a conserved Drosophila gene required fro mitochondrial subcellular localization, interacts genetically with parkin. Disease Models and Mechanisms. 2009, 2 (9–10): 490-499.PubMedPubMed CentralView ArticleGoogle Scholar
- Motley AM, Hettema EH: Yeast peroxisomes multiply by growth and division. The Journal of Cell Biology. 2007, 178 (3): 399-410. 10.1083/jcb.200702167.PubMedPubMed CentralView ArticleGoogle Scholar
- Dohm JA, Lee SJ, Hardwick JM, Hill RB, Gittis AG: Cytosolic domain of the human mitochondrial fission protein fis1 adopts a TPR fold. Proteins: Structure, Function, and Bioinformatics. 2004, 54 (1): 153-156.View ArticleGoogle Scholar
- Motley AM, Ward GP, Hettema EH: Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p. Journal of Cell Science. 2008, 121 (10): 1633-1640. 10.1242/jcs.026344.PubMedPubMed CentralView ArticleGoogle Scholar