Table 1 Characterization of ten microsatellite loci for brown mussel Perna perna
Locus name (Genebank no.) | Primer sequences | Repeat motif | Clone size (bp) | PCR annealing (°C) | A | Size range (bp) | HE | HO | FIS | Cross amplification (positive/total) |
|---|---|---|---|---|---|---|---|---|---|---|
Perper-01 | F-TGGAACTTAGGGCCTTCCTC | (AC)13 | 176 | 56 | 9 | 158-183 | 0.8075 | 0.45 | 0.46311** | 3/5 |
(JX183697) | R-TCCAATTCTGTGAAATCATTGAC | |||||||||
Perper-02 | F-CCCGGTTTTAAGTGGTAGATG | (GT)15 | 113 | 57 | 6 | 110-137 | 0.3112 | 0.3000 | 0.06173 | 0/5 |
(JX183698) | R-AGCAAACGAAGGACAAATCG | |||||||||
Perper-05 | F-TCAGTGCCGCGTGATAATAC | (TG)15 | 127 | 59 | 16 | 111-150 | 0.885 | 1.000 | −0.10465 | 4/5 |
(JX183699) | R-TCCAATTACGTTTGTTTTTGC | |||||||||
Perper-08 | F-AATGTTCAATGTCGACAGACTATG | (ACAG)7 | 129 | 56 | 5 | 110-126 | 0.7604 | 0.5789 | 0.26394* | 0/5 |
(JX183700) | R-TTCTGAAGCACTGGATGTGG | |||||||||
Perper-11 | F-TTAACGTTGAAATCCGTGAGG | (GT)9…(GT)13 | 133 | 58 | 16 | 70-167 | 0.9044 | 0.4667 | 0.51000** | 4/5 |
(JX183701) | R-CATTCCAATCCCACGCATAC | |||||||||
Perper-16 | F-TGTGATTTAAAGTTGGACTTGTTTC | (AC)26 | 134 | 56 | 8 | 104-130 | 0.5606 | 0.2353 | 0.60000** | 0/5 |
(JX183702) | R-TGATTGGATCAAAATTAAACGTG | |||||||||
Perper-20 | F-ATGTCAATGTGCACAACACG | (ACAG)15 | 245 | 55 | 27 | 202-382 | 0.9525 | 0.9000 | 0.08065 | 0/5 |
(JX183703) | R-CGTGTATTGGCGACTTTTTATC | |||||||||
Perper-26 | F-CACCACCCTTACAAAGACGTG | (AC)11 | 102 | 53 | 11 | 92-113 | 0.845 | 0.7500 | 0.13767 | 5/5 |
(JX183704) | R-TTTCACTTGGCGATTAGTATGC | |||||||||
Perper-27 | F-CCCAATTTAACGGGAACAAC | (AC)27 | 167 | 56 | 13 | 103-193 | 0.8449 | 0.9474 | −0.09459 | 4/5 |
(JX183705) | R-TTATCATCACCACTTTAAGTTACCC | |||||||||
Perper-29 | F-TTCCATTTCTAGACATCTCTGTCG | (AC)11 | 80 | 56 | 5 | 64-78 | 0.6763 | 0.95 | −0.38314 | 5/5 |
(JX183706) | R-TCAGGTGACAGCAGCCTGAC |