Bacterial and fungal chitinase chiJ orthologs evolve under different selective constraints following horizontal gene transfer
© Ubhayasekera and Karlsson; licensee BioMed Central Ltd. 2012
Received: 8 August 2012
Accepted: 22 October 2012
Published: 24 October 2012
Certain bacteria from the genus Streptomyces are currently used as biological control agents against plant pathogenic fungi. Hydrolytic enzymes that degrade fungal cell wall components, such as chitinases, are suggested as one possible mechanism in biocontrol interactions. Adaptive evolution of chitinases are previously reported for plant chitinases involved in defence against fungal pathogens, and in fungal chitinases involved in fungal-fungal interactions. In this study we investigated the molecular evolution of chitinase chiJ in the bacterial genus Streptomyces. In addition, as chiJ orthologs are previously reported in certain fungal species as a result from horizontal gene transfer, we conducted a comparative study of differences in evolutionary patterns between bacterial and fungal taxa.
ChiJ contained three sites evolving under strong positive selection and four groups of co-evolving sites. Regions of high amino acid diversity were predicted to be surface-exposed and associated with coil regions that connect certain α-helices and β-strands in the family 18 chitinase TIM barrel structure, but not associated with the catalytic cleft. The comparative study with fungal ChiJ orthologs identified three regions that display signs of type 1 functional divergence, where unique adaptations in the bacterial and fungal taxa are driven by positive selection.
The identified surface-exposed regions of chitinase ChiJ where sequence diversification is driven by positive selection may putatively be related to functional divergence between bacterial and fungal orthologs. These results show that ChiJ orthologs have evolved under different selective constraints following the horizontal gene transfer event.
KeywordsChitinase Functional divergence Homology modelling Horizontal gene transfer Hypocrea Protein evolution Streptomyces Trichoderma
Chitin is an insoluble linear β-1,4-linked polymer of N-acetylglucosamine which is an essential structural component of fungal cell walls. Chitinases (EC 22.214.171.124) are hydrolytic enzymes that catalyse the degradation of chitin and are classified into glycoside hydrolase families 18 and 19, based on amino acid sequence similarity [1, 2]. These two families possess different three-dimensional structural folds as well as modes of action. Family 18 chitinases is based on a triosephosphate isomerase (TIM) barrel structure and use a substrate-assisted double-displacement with net retention of the configuration of the anomeric carbon during the catalysis .
Biological control of pathogenic fungi causing diseases on important crop plants is an attractive alternative to the extensive use of chemical fungicides in modern agriculture, which may have negative environmental impacts and result in evolution of fungicide resistance in pathogen populations. Certain bacteria from the genus Streptomyces and fungi from the genera Hypocrea (anamorph Trichoderma) and Bionectria (anamorph Clonostachys) are currently used as biological control agents. The biocontrol ability of these microbes are suggested to be the result of several mechanisms that act synergistically, including direct antagonism by toxins and cell wall degrading enzymes (including chitinases), induced resistance in plants, or competition for nutrients and space .
Streptomyces bacteria are well known for their production of secreted hydrolytic enzymes and secondary metabolites , illustrating their ecological niche as ubiquitous soil bacteria. The genetically best studied species is S. coelicolor which is reported to possess 12 different chitinase genes, ten family 18 members (chiA, B, C, D, E, H, I, J, K, L) and two family 19 members (chiF, G) . The ten family 18 chitinases can be phylogenetically divided into subfamilies A, B and C, and subgroups II, IVa and VI [6, 7]. Seven genes, chiA, B, C, D, F, I and J, are reported to be induced to varying degrees in the presence of colloidal chitin [6, 8], while studies of enzymatic activity are reported for S. coelicolor ChiA, C, D, F and G [6, 9]. Recently, chiJ is shown to have an ortholog in Hypocrea species (chi18-15/chit36) and in Bionectria ochroleuca (ech37), acquired through horizontal gene transfer (HGT) [7, 10, 11].
Certain Hypocrea and Bionectria species are mycoparasitic, i.e. they have the ability to parasitize other fungi and use them as a source of nutrients. Studies of gene family evolution show that the mycoparasitic lifestyle has resulted in selection for increased number of cell wall degrading chitinases in Hypocrea. In addition, certain Hypocrea chitinases evolve rapidly in a manner consistent with co-evolutionary interactions between parasite and host , similar to certain plant chitinases involved in defence against fungal pathogens [12, 13].
In this study we investigate the molecular evolution of Streptomyces chitinase ChiJ, under the hypothesis that its involvement in interactions with soil fungi have resulted in sequence diversification between taxa. In addition, the HGT event presents an opportunity for a comparative investigation of the molecular fate of ChiJ orthologs evolving in bacterial and fungal species, respectively. We hypothesize that bacterial and fungal ChiJ orthologs evolve differently due to the fundamentally different organismal environments.
Materials and methods
Identification of genes and phylogenetic analysis
Streptomyces genome sequences available at the National Center for Biotechnological Information (NCBI) were screened for the presence of chitinase chiJ [GenBank:NP_626743], DNA recombinase A recA [GenBank:NP_629894] and DNA polymerase III subunit beta dnaN [GenBank:NP_628065] using BLAST . GenBank  sequences were screened with ChiJ using BLAST. Sequences were aligned with Clustal X . Phylogenetic analysis was performed using maximum likelihood methods implemented in PhyML-aLRT ver. 2.4.5 [17, 18]. The JTT amino acid substitution model  was used, the proportion of invariable sites was set to 0, one category of substitution rate was used and gaps were treated as unknown characters. The starting tree to be refined by the maximum likelihood algorithm was a distance-based BIONJ tree estimated by the program . Statistical support for phylogenetic grouping was assessed by approximate likelihood-ratio tests based on a Shimodaira-Hasegawa-like procedure  and by bootstrap analysis (1000 resamplings).
Similar sequences were located by BLAST in the protein entries of GenBank and aligned using Clustal W . Similar chitinase catalytic module structures (Additional file 1) were obtained from the Protein Data Bank (PDB ), then superimposed and compared with the program O . Multiple sequence alignments were used to identify the best pair-wise alignment of the Streptomyces sp. Mg1 [GenBank:ZP_04999219] enzyme with that of Lactococcus lactis subsp. lactis. This pair-wise alignment was the basis of creating a homology model, with PDB entry [PDB:3IAN] (L. lactis subsp. lactis) as the template in the program SOD . The model was adjusted in O, using rotamers that would improve packing in the interior of the protein. The model is available upon request from the authors. The figure was prepared using O, MOLSCRIPT  and Molray .
Reverse conservation analysis
Amino acid diversity in sequence alignments were analysed using Reverse Conservation Analysis (RCA). Amino acid sequences were aligned by Clustal X  and amino acid conservation at each position was analysed by RCA analysis as described by Lee (2008) . In short, Rate4Site (Version 2.01) was used to calculate the degree of conservation (S score) for each amino acid position using the empirical Bayesian method [27, 28]. A sliding window-average (n = 7) S score was plotted (W mean score) and significant peaks were defined by intensity (I) values of 0.5, as recommended by Lee (2008) .
The rate of non-synonymous (dN) and synonymous (dS) substitutions at each codon, and identification of sites evolving under positive or negative selection, was determined using random effects maximum likelihood models (REL)  implemented in the HyPhy software package  through the Datamonkey webserver . The optimal nucleotide substitution model was estimated for each gene separately  and included the following modifications to the general reversible nucleotide model  for chiJ: C↔G: R1, C↔T: RAC, G↔T: RAT; for recA: A↔C: R1, A↔T: R1, C↔G: RCG, C↔T: RCT, G↔T: RGT, and for dnaN: A↔C: R1, A↔T: R1, C↔G: RCG, G↔T: R1. A Bayes factor value ≥ 50 (default) was used as an indication of strong positive selection at a site, while values between 10 and 49 were considered to indicate weak support of positive selection .
Identification of co-evolving sites was done using the Spidermonkey/BGM program  implemented in the HyPhy software package  through the Datamonkey webserver . The same nucleotide substitution models as in REL analysis was used, global dN/dS values were estimated by the program, ambiguous characters were averaged, a two-parent, directed network was used and sites were selected based on non-synonymous branch counts (threshold ≥ 3). A posterior probability value ≥ 0.5 (default) was used as the definition of association between sites.
Identification of chiJ orthologs
Homology modelling of ChiJ
Distribution of amino acid diversity
Patterns of amino acid diversity can provide important information about the type of selective constraints that act on different parts of an enzyme. In distantly related orthologous sequences only functionally important regions are expected to be conserved between species, while other regions are expected to have a low degree of conservation due to accumulation of neutral mutations. In the case of closely related orthologs, such as ChiJ in the current work, a high degree of conservation is expected for most positions as the limited time since speciation has not allowed sufficient sequence diversification by genetic drift. However, as outlined by Lee (2008) , residues important for enzyme properties may be expected to display higher diversity than other positions in closely related orthologs due to selection for modified enzymatic properties between species.
Evolutionary patterns among sites
High sequence diversity in certain regions and sites between closely related orthologs may be the result of either low selective constraint, i.e. mutations do not alter enzyme function in a significant manner, or to positive selection where certain mutations provide a selective advantage that improve enzyme function. The ratio between non-synonymous and synonymous substitutions is expected to approach 1 in the case of low selective constraints and be > 1 in the case of positive selection.
Positively selected sites in ChiJ
Amino acid position
For comparative purposes, REL analysis was performed on sequence alignments of two Streptomyces genes whose functions were assumed to be independent from chitin degradation or microbial interactions, recA and dnaN. No rate class was estimated to have a dN/dS value above 1 for either recA or dnaN.
Co-evolution between sites
Amino acid residues in an enzyme can interact functionally to perform a specific function, or structurally to stabilize protein structures. Co-evolution between sites can result from selection for mutations that compensate for other, slightly deleterious mutations, to preserve protein function or structure. Identification of co-evolving sites in a protein may thus provide information about the boundaries of functional or structural domains. Functional domains can be especially difficult to define as functionality may depend on residues that are distributed over the whole protein.
Co-evolving sites in ChiJ
Amino acid position 1
Amino acid position 2
Comparison between chiJ and fungal orthologs
Fungal species of the genera Hypocrea and B. ochroleuca were previously shown to possess an ortholog to chiJ (chi18-15/chit36 and ech37 respectively), acquired through HGT from its actinobacterial origin [7, 10, 11]. An alignment of ChiJ (excluding the first 36 amino acids) from S. coelicolor and S. sp. Mg1, chi18-15 from H. jecorina, CHIT36 from H. atroviridis and Ech37 from B. ochroleuca shows that no insertions or deletions have occurred since the HGT event (data not shown). However, when comparing regions exhibiting high amino acid diversity from the RCA analysis in the current paper with a previous analysis on the fungal (Hypocrea) ChiJ orthologs , several differences were observed. ChiJ regions I and IV both exhibited high amino acid variation between Streptomyces species and contained rapidly evolving sites, while these regions were highly conserved in the fungal counterparts (Figure 3). The same trend was observed for ChiJ regions VI and VII (Figure 3). On the other hand, one region (region A) in the fungal chi18-15/CHIT36 protein alignment was highly variable between fungal species at the amino acid level, but conserved between bacterial ChiJ orthologs (Figure 3).
Adaptive evolution of chitinases have previously been shown for plant chitinases involved in defence against fungal pathogens [12, 13], and in fungal chitinases involved in mycoparasitic interactions . The common theme between these examples is that parasite – host interaction seem to exert selection for rapid modification of the involved enzymes in an arms race fashion. Enzyme modification may thus partly account for variation in virulence or disease resistance in the parasite and host, respectively.
The biological function of ChiJ is suggested to include degradation of exogenous chitin for nutrient acquisition and aggressive interactions with soil fungi. Evolution of ChiJ may be influenced by the presence of several additional chitinases in certain Streptomyces species . Conservation of gene duplications may be favoured if high levels of a specific chitinase is needed. However, the substantial divergence between ChiA, ChiB and ChiJ that is evident from our phylogenetic analysis suggest that these paralogous proteins represent isozymes with different functional properties that may act synergistically in chitin degradation. Presence of a fungal chiJ ortholog in both Hypocrea spp. and in B. ochroleuca suggests that the HGT event preceded the split of these species. These species belong to the same order (Hypocreales) but different families, so the HGT event cannot be considered to be recent. B. ochroleuca and certain Hypocrea species are mycoparasitic, and thus Ech37/CHIT36 are suggested to participate in degradation of the cell wall of antagonistic fungal species [36, 37]. Expression data show that ech37 in B. ochroleuca is indeed induced during interaction with Botryotinia fuckeliana on strawberry leaves , while chit36 in T. asperellum is induced during plate confrontation with Rhizoctonia solani.
No region of high amino acid diversity or sites showing signs of adaptive evolution constitute parts of the catalytic cleft of ChiJ. This suggests that adaptation for changes in substrate-specificity between different Streptomyces species is not the primary selection force for ChiJ evolution. This conclusion is further supported by the localization of all residues predicted to be important for catalysis and substrate-binding to conserved regions. In addition, Hypocrea spp. Chi18-15/CHIT36 interspecific sequence variability is also reported to localize to other parts of the enzyme than the catalytic cleft . This may indicate that the primary substrate of ChiJ and Chi18-15/CHIT36 are of similar composition, presumably fungal cell wall material.
Instead, regions with high amino acid diversity and sites under positive selection in ChiJ coincided with coil regions that connect certain α-helices and β-strands in the family 18 chitinase TIM barrel structure. This result is not surprising as the basic architecture of the TIM barrel is highly conserved between species, while surface loops may vary considerably due to low selective constraints which results in accumulation of neutral mutations. However, some of these surface loops will also be determinants of enzyme properties and interactions with other proteins, and may thus be targets for diversifying selection. Therefore, a major challenge in studies of enzyme evolution is to determine if the observed sequence diversity between orthologs is due to low selective constraints or due to positive selection. Identification of positive selection in a gene strongly implies that these changes are the result from diversifying selection and that the involved sites are connected to protein function.
In the current work, regions I, II, IV, V, VI and VII all contain sites evolving under positive selection. Hence, the most valid interpretation is that the high amino acid diversity between Streptomyces species in these regions is the results from diversifying selection and may influence functional properties of ChiJ. The localization of the variable parts to certain coil-regions predicts changes in enzyme architecture between species. Another observation is that two pairs of co-evolving sites are located on opposite sides of the enzyme, indicating that they participate in the same functional domain, i.e. slightly deleterious mutations in the substrate side of ChiJ may be compensated by other mutations on the products side, or vice versa. The surface-exposure of all seven regions of high amino acid diversity may suggest that interactions with environmental factors are the cause of the observed interspecific variability. No single factor can be identified at this stage, but may include interactions with chitinase inhibitors that are produced from soil fungi , plants , or from Streptomyces itself . ChiJ may also interact and evolve together with chitin-binding proteins that enhance the efficiency of the degradation process [42, 43]. A comparison of the enzymatic activities of S. coelicolor ChiA, ChiC, ChiD and ChiF showed that both pH optima and hydrolytic activity against soluble or crystalline chitin differed substantially between the enzymes . Sites evolving under positive selection in ChiJ are candidates for targeted mutagenesis to elucidate mutational effects on enzyme properties.
The comparison between ChiJ and its fungal orthologs identified two regions that display high amino acid diversity in combination with sites evolving under positive selection in Streptomyces but not in fungi (regions I and IV), and one region of high sequence diversity in the fungal orthologs only (region A). This difference in amino acid conservation/diversity patterns is a hallmark of selection for type 1 functional divergence ; if the observed diversity in these regions is due to low selective constraints we would expect a similar level of diversity in both bacterial and fungal orthologs. Regions I and IV may thus represent areas on the ChiJ surface that contain structural features that specifically interact with environmental factors, such as inhibitors or chitin-binding helper proteins, present in the ecological niche of Streptomyces but not Hypocrea. Similarly, region A with high amino acid diversity between fungal orthologs may represent adaptations after the HGT event towards fungal preferences. In fact, this particular region was previously identified in Hypocrea chi18-15/CHIT36 to contain sites evolving under positive selection . Furthermore, that study also shows discrete differences in amino acid conservation pattern between Hypocrea clades in this region; species in clades Rufa and Pachybasioides display high interspecific amino acid diversity in this region compared to a conserved interspecific pattern in other clades .
In summary, we show that several surface-exposed regions of Streptomyces chitinase ChiJ evolves rapidly and that this divergence is driven by selection for adaptive modifications. This shows that ChiJ function, whether it be interactions with soil fungi or nutrient acquisition, is important in the ecological niche of Streptomyces spp. We also identify three regions that display signs of type 1 functional divergence, where unique adaptations in the bacterial and fungal taxa are driven by positive selection. These results are noteworthy as it shows that different regions of the ChiJ orthologs have evolved under different selective constraints following the HGT event. The identified regions may thus influence functional properties of ChiJ.
Availability of supporting data
The data set supporting the results of this article is included within the article (and its additional files).
Horizontal gene transfer
National center for biotechnological information
Protein data bank
Reverse conservation analysis
Random effects maximum likelihood
Contributions to Streptomyces genome sequence data to NCBI are gratefully acknowledged. This work was supported by grant CTS 08:189 from the Carl Trygger Foundation (MK), by the Royal Swedish Academy of Sciences (MK) and by DANSCATT (WU). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Henrissat B: A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J. 1991, 280: 309-316.PubMedPubMed CentralView ArticleGoogle Scholar
- Henrissat B, Bairoch A: New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J. 1993, 293: 781-788.PubMedPubMed CentralView ArticleGoogle Scholar
- Tews I, van Scheltinga ACT, Perrakis A, Wilson KS, Dijkstra BW: Substrate-assisted catalysis unifies two families of chitinolytic enzymes. J Am Chem Soc. 1997, 119: 7954-7959. 10.1021/ja970674i.View ArticleGoogle Scholar
- Harman GE, Howell CR, Viterbo A, Chet I, Lorito M: Trichoderma species - Opportunistic, avirulent plant symbionts. Nature Rev Microbiol. 2004, 2: 43-56. 10.1038/nrmicro797.View ArticleGoogle Scholar
- Hodgson DA: Primary metabolism and its control in Streptomycetes: A most unusual group of bacteria. Adv Microb Physiol. 2000, 42: 47-238.PubMedView ArticleGoogle Scholar
- Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N, Miyashita K, Watanabe T: Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor A3(2). Biosci Biotech Bioch. 2006, 70: 988-998. 10.1271/bbb.70.988.View ArticleGoogle Scholar
- Karlsson M, Stenlid J: Evolution of family 18 glycoside hydrolases: Diversity, domain structures and phylogenetic relationships. J Mol Microbiol Biotechnol. 2009, 16: 208-223. 10.1159/000151220.PubMedView ArticleGoogle Scholar
- Saito A, Ishizaka M, Francisco PB, Fujii T, Miyashita K: Transcriptional co-regulation of five chitinase genes scattered on the Streptomyces coelicolor A3(2) chromosome. Microbiol Sgm. 2000, 146: 2937-2946.View ArticleGoogle Scholar
- Heggset EB, Hoell IA, Kristoffersen M, Eijsink VGH, Vårum KM: Degradation of chitosans with chitinase G from Streptomyces coelicolor A3(2): Production of chito-oligosaccharides and insight into subsite specificities. Biomacromolecules. 2009, 10: 892-899. 10.1021/bm801418p.PubMedView ArticleGoogle Scholar
- Ihrmark K, Asmail N, Ubhayasekera W, Melin P, Stenlid J, Karlsson M: Comparative molecular evolution of Trichoderma chitinases in response to mycoparasitic interactions. Evol Bioinform. 2010, 6: 1-26.Google Scholar
- Mamarabadi M, Jensen B, Lübeck M: Three endochitinase-encoding genes identified in the biocontrol fungus Clonostachys rosea are differentially expressed. Curr Genet. 2008, 54: 57-70. 10.1007/s00294-008-0199-5.PubMedView ArticleGoogle Scholar
- Bishop JG, Dean AM, Mitchell-Olds T: Rapid evolution in plant chitinases: Molecular targets of selection in plant-pathogen coevolution. P Natl Acad Sci USA. 2000, 97: 5322-5327. 10.1073/pnas.97.10.5322.View ArticleGoogle Scholar
- Tiffin P: Comparative evolutionary histories of chitinase genes in the genus Zea and family Poaceae. Genetics. 2004, 167: 1331-1340. 10.1534/genetics.104.026856.PubMedPubMed CentralView ArticleGoogle Scholar
- Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.PubMedPubMed CentralView ArticleGoogle Scholar
- Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL: GenBank. Nucleic Acids Res. 2008, 36: D25-D30. 10.1093/nar/gkn320.PubMedPubMed CentralView ArticleGoogle Scholar
- Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: Clustal W and clustal X version 2.0. Bioinformatics. 2007, 23: 2947-2948. 10.1093/bioinformatics/btm404.PubMedView ArticleGoogle Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704. 10.1080/10635150390235520.PubMedView ArticleGoogle Scholar
- Anisimova M, Gascuel O: Approximate likelihood-ratio test for branches: A fast, accurate, and powerful alternative. Syst Biol. 2006, 55: 539-552. 10.1080/10635150600755453.PubMedView ArticleGoogle Scholar
- Jones DT, Taylor WR, Thornton JM: The rapid generation of mutation data matrices from protein sequences. Comp Appl Biosci. 1992, 8: 275-282.PubMedGoogle Scholar
- Thompson JD, Higgins DG, Gibson TJ: Clustal-W - Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.PubMedPubMed CentralView ArticleGoogle Scholar
- Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE: The Protein Data Bank. Nucleic Acids Res. 2000, 28: 235-242. 10.1093/nar/28.1.235.PubMedPubMed CentralView ArticleGoogle Scholar
- Jones TA, Zou JY, Cowan SW, Kjeldgaard M: Improved methods for building protein models in electron-density maps and the location of errors in these models. Acta Crystallogr Sec A. 1991, 47: 110-119. 10.1107/S0108767390010224.View ArticleGoogle Scholar
- Kleywegt GJ, Zou JY, Kjeldgaard M, Jones TA: Around O. International tables for crystallography, Vol F Crystallography of biological macromolecules. Edited by: Rossmann MG, Arnold E. 2001, Kluwer Academic, Dordrecht, 353-356. 366–367Google Scholar
- Kraulis PJ: Molscript - a program to produce both detailed and schematic plots of protein structures. J Appl Crystallogr. 1991, 24: 946-950. 10.1107/S0021889891004399.View ArticleGoogle Scholar
- Harris M, Jones TA: Molray - a web interface between O and the POV-Ray ray tracer. Acta Crystallogr Sec D. 2001, 57: 1201-1203.View ArticleGoogle Scholar
- Lee T: Reverse conservation analysis reveals the specificity determining residues of cytochrome P450 family 2 (CYP 2). Evol Bioinform. 2008, 4: 7-16.Google Scholar
- Mayrose I, Graur D, Ben-Tal N, Pupko T: Comparison of site-specific rate-inference methods for protein sequences: Empirical Bayesian methods are superior. Mol Biol Evol. 2004, 21: 1781-1791. 10.1093/molbev/msh194.PubMedView ArticleGoogle Scholar
- Pupko T, Bell R, Mayrose I, Glaser F, Ben-Tal N: Rate4Site: an algorithmic tool for the identification of functional regions in proteins by surface mapping of evolutionary determinants within their homologues. Bioinformatics. 2002, 18: S71-S77. 10.1093/bioinformatics/18.suppl_1.S71.PubMedView ArticleGoogle Scholar
- Pond SLK, Frost SDW: Not so different after all: A comparison of methods for detecting amino acid sites under selection. Mol Biol Evol. 2005, 22: 1208-1222. 10.1093/molbev/msi105.View ArticleGoogle Scholar
- Pond SLK, Frost SDW, Muse SV: HyPhy: hypothesis testing using phylogenies. Bioinformatics. 2005, 21: 676-679. 10.1093/bioinformatics/bti079.PubMedView ArticleGoogle Scholar
- Pond SLK, Frost SDW: Datamonkey: rapid detection of selective pressure on individual sites of codon alignments. Bioinformatics. 2005, 21: 2531-2533. 10.1093/bioinformatics/bti320.PubMedView ArticleGoogle Scholar
- Pond SLK, Frost SDW: A simple hierarchical approach to modeling distributions of substitution rates. Mol Biol Evol. 2005, 22: 223-234.PubMedView ArticleGoogle Scholar
- Poon A, Lewis F, Kosakovsky Pond S, Frost S: An evolutionary-network model reveals stratified interactions in the V3 loop of the HIV-1 envelope. PLoS Comp Biol. 2007, 3: e231-10.1371/journal.pcbi.0030231.View ArticleGoogle Scholar
- Saito A, Fujii T, Miyashita K: Distribution and evolution of chitinase genes in Streptomyces species: Involvement of gene-duplication and domain-deletion. Antonie Van Leeuwenhoek. 2003, 84: 7-15. 10.1023/A:1024463113606.PubMedView ArticleGoogle Scholar
- Bokma E, Rozeboom HJ, Sibbald M, Dijkstra BW, Beintema JJ: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis. Eur J Biochem. 2002, 269: 893-901. 10.1046/j.0014-2956.2001.02721.x.PubMedView ArticleGoogle Scholar
- Viterbo A, Haran S, Friesem D, Ramot O, Chet I: Antifungal activity of a novel endochitinase gene (chit36) from Trichoderma harzianum Rifai TM. FEMS Microbiol Lett. 2001, 200: 169-174. 10.1111/j.1574-6968.2001.tb10710.x.PubMedView ArticleGoogle Scholar
- Mamarabadi M, Jensen B, Jensen DF, Lübeck M: Real-time RT-PCR expression analysis of chitinase and endoglucanase genes in the three-way interaction between the biocontrol strain Clonostachys rosea IK726, Botrytis cinerea and strawberry. FEMS Microbiol Lett. 2008, 285: 101-110. 10.1111/j.1574-6968.2008.01228.x.PubMedView ArticleGoogle Scholar
- Viterbo A, Montero M, Ramot O, Friesem D, Monte E, Llobell A, Chet I: Expression regulation of the endochitinase chit36 from Trichoderma asperellum (T. harzianum T-203). Curr Genet. 2002, 42: 114-122. 10.1007/s00294-002-0345-4.PubMedView ArticleGoogle Scholar
- Arai N, Shiomi K, Yamaguchi Y, Masuma R, Iwai Y, Turberg A, Kolbl H, Omura S: Argadin, a new chitinase inhibitor, produced by Clonostachys sp FO-7314. Chem Pharm Bull. 2000, 48: 1442-1446. 10.1248/cpb.48.1442.PubMedView ArticleGoogle Scholar
- Hurtado-Guerrero R, van Aalten DMF: Structure of Saccharomyces cerevisiae chitinase 1 and screening-based discovery of potent inhibitors. Chem Biol. 2007, 14: 589-599. 10.1016/j.chembiol.2007.03.015.PubMedView ArticleGoogle Scholar
- Sakuda S, Isogai A, Matsumoto S, Suzuki A: Search for microbial insect growth-regulators .2. Allosamidin, a novel insect chitinase inhibitor. J Antibiot. 1987, 40: 296-300. 10.7164/antibiotics.40.296.PubMedView ArticleGoogle Scholar
- Vaaje-Kolstad G, Horn SJ, van Aalten DMF, Synstad B, Eijsink VGH: The non-catalytic chitin-binding protein CBP21 from Serratia marcescens is essential for chitin degradation. J Biol Chem. 2005, 280: 28492-28497. 10.1074/jbc.M504468200.PubMedView ArticleGoogle Scholar
- Saito A, Miyashita K, Biukovic G, Schrempf H: Characteristics of a Streptomyces coelicolor A3(2) extracellular protein targeting chitin and chitosan. Appl Environ Microb. 2001, 67: 1268-1273. 10.1128/AEM.67.3.1268-1273.2001.View ArticleGoogle Scholar
- Cole MF, Gaucher EA: Exploiting models of molecular evolution to efficiently direct protein engineering. J Mol Evol. 2011, 72: 193-203. 10.1007/s00239-010-9415-2.PubMedPubMed CentralView ArticleGoogle Scholar
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