Table 3 Gene identification and primers sequences used in the qPCR analyses
From: Validation of reference genes from Eucalyptus spp. under different stress conditions
Gene | Primer | Primer sequence (5’-3’) | Amplicon (bp)* | Amplification efficiency (%)** |
|---|---|---|---|---|
Elongation factor-1α | Forward | CCTGTCCTTGATTGTCACACTTCC | 130 | 110 |
Reverse | CCATTCCAGCATCACCGTTCTTC | |||
Ubiquitin (E. gobulus) | Forward | TCCGTCAAAAGCGAACAGA | 173 | 97 |
Reverse | CATTTCCCTCCAGATTACCC | |||
Ubiquitin (E. urograndis) | Forward | GGACTTTCGTTCGTTTTGGT | 107 | 97 |
Reverse | GTGATTTGGGGAGGGTTTG | |||
Actin | Forward | AGATGACCCAGATTATGTTTGAGACCTTC | 122 | 97 |
Reverse | ACCATCACCAGAATCCAACACAATACC | |||
SAND protein | Forward | TGGGTCACACAGGATTTTGA | 130 | 100 |
Reverse | CTCCCAGCAAAAAGATCTCG | |||
Isocitrate dehydrogenase-NADP | Forward | AGTTTGAGGCTGCTGGAATC | 100 | 103 |
Reverse | CTTGCATGCCCACACATAAC | |||
Histone H2B | Forward | AACAAGAAGCCCACCATCAC | 142 | 96 |
Reverse | ACAACTTCCTCCTCGCTCAC | |||
α-Tubulin | Forward | CCAGTGAACAAATGCCCTCT | 92 | 107 |
Reverse | TGATCAGCAACAACACAGCA | |||
Ribossomal 18S | Forward | CATGGCCGTTCTTAGTTGGT | 71 | 95 |
Reverse | TAGCAGGCTGAGGTCTCGTT | |||
β-Tubulin | Forward | GATGGGGACGCTATTGATTT | 225 | 100 |
Reverse | CTTGGGTTGATGAGTTTCAGG |