Table 2 DNA concentration ng/μL, presented as median (maximum – minimum) measured using three different measurement methods
From: DNA extract characterization process for microbial detection methods development and validation
Extraction method | UV spectroscopy | qPCR | Fluorometry |
|---|---|---|---|
Reference | 524.4 (1502.2 − <LOQ*) | 144.2 (487.7 − 0.02) | 84.2 (460.0 − <LOQ) |
PrecipB | 61.1 (178.3 − 3.8) | 101.5 (149.4 − 0.00) | 51.8 (178.4 − 3.8) |
ChemLysis | 16.5 (110.2 − <LOQ) | 2.1 (47.7 − 0.19) | 2.2 (55.2 − <LOQ) |
PrecipG | 14.9 (44.4 − <LOQ) | 8.4 (94.5 − 0.17) | 6.2 (29.6 − <LOQ) |
PrecipS | 4.0 (12.0 − <LOQ) | 1.5 (6.1 − 0.01) | 1.4 (9.5 − <LOQ) |
MagBeads | 4.3 (7.6 − <LOQ) | 0.2 (1.3 − <LOQ) | 0.4 (2.4 − <LOQ) |