Table 4 M. smithii real-time PCR detection in 41 diarrheal stool specimens, before and after lyophilization (Ct value)
From: Optimized microbial DNA extraction from diarrheic stools
Sample n° | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Without lyophilization | NA | 25.55 | NA | 18.87 | 39.33 | 36.94 | 37.84 | 37.69 | 31.95 | 38.51 | 39.22 | 40.32 | 24.18 | 36.14 | NA |
With lyophilization | 38.98 | 18.82 | 35.20 | 16.29 | 25.62 | 23.21 | 26.54 | 28.51 | 24.41 | 23.96 | 25.2 | 23.67 | 23.65 | 23.98 | 17.5 |
Sample n° | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 |
Without lyophilization | 34.83 | NA | NA | 33.91 | NA | 36.45 | 18.55 | NA | 37.05 | 22.02 | 39.05 | 19.28 | 33.16 | 19.25 | NA |
With lyophilization | 21.53 | 22.47 | 19.34 | 27.19 | 18.30 | 27.48 | 21.69 | 24.49 | 24.14 | 20.53 | 23.88 | 19.18 | 13.92 | 19.27 | 24.44 |
Sample n° | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | ||||
Without lyophilization | NA | NA | 35.67 | 22.78 | NA | 19.2 | 15.97 | 19.59 | 13.92 | 17.98 | 18.4 | ||||
With lyophilization | 18.08 | 29.99 | NA | 14.52 | 12.63 | 19.23 | 15.32 | 18.52 | 13.57 | 18.25 | 18.27 |