Table 1 Name, symbol, function, primer sequences and accession number for the housekeeping genes and the target gene

Actin 5C

Actin

Cytoskeletal structural protein

F 5'-CATCAGGGAGTCATGGTCGGCA-3'

JQ086312

R 5'-GCATACAGGTCTTTACGGATGTCC-3'

Elongation factor 1 alpha

EF1-a

Protein synthesis

F 5'-TTCGAGAAGGAAGCCCAGGARATGG-3'

JQ086311

R 5'-CACDGAYGGACCAATCAGCACAYCT-3'

Glyceraldehyde-3-phosphate dehydrogenase

GAPDH

Glycolysis

F 5'-ATGTCDAAGATYGGWATCAACGG-3'

KC149901

R 5'-TTAGTCYTTKGACTGCATG-3'

Armadillo

Arm

Cell adhesion

F 5'-ATGGARGARATHGTNGARGGAAC-3'

KC149902

R 5'-TTGTARTCYTGNGGYTTRTCYTC-3'

Ribosomal protein L32

RpL32

Ribosomal structural constituent

F 5'-AARCCNAARGGNATYGACAAYAG-3'

KC149903

R 5'-CRCARTAYTTSCKRTTMTGCATCAT-3'

Succinate dehydrogenase

SDHa

Energy metabolism

F 5'-TGGCAGTGGCAYATGTAYGAY-3'

KC149904

R 5'-GTYTGCATNGTYTTYTGCAT-3'

Annexin IX

AnnIX

Cell differentiation, membrane fusion

F 5'-AARTGYACYCCHACRGTRTAYCC-3'

KC149905

R 5'-TTRATRTCMSCYARRTCNATYTC-3'

a-tubulin

Tub

Cytoskeletal structural protein

F 5'-ATGCGTGARTGTATHTCRRTBCA-3'

KC149906

R 5'-GARTCCATNCCNACYTCYTCRTA-3'

Follicle cell protein 3c

Fcp 3c

Vitellogenesis

F 5'-TGYRYHTGTGGARTGTTYTTRA-3'

KC149907

R 5'-TCYTTRCARCARTAYTCYCKWCC-3'