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Table 1 Stability and pairwise variation of housekeeping gene expression in reproductive tissues after mitogen stimulation

From: Housekeeping gene expression stability in reproductive tissues after mitogen stimulation

 

Stability (M)

Pairwise variation

GAPDH

ACTB

RNA18S1

(V2/3)

Umbilical cord

 

(n=3 with duplicates)

LPS

0.110*

0.095*

0.151

0.050

PHA

0.092*

0.094*

0.138

0.045

Fetal membranes

 

(n=3 with duplicates)

LPS

0.055*

0.061*

0.082

0.027

PHA

0.053*

0.050*

0.065

0.021

Placenta

 

(n=3 with duplicates)

LPS

0.105*

0.128

0.097*

0.041

PHA

0.088*

0.105

0.098*

0.032

Cervix

 

(n=3 with duplicates)

LPS

0.089*

0.102*

0.120

0.038

PHA

0.079*

0.092*

0.113

0.037

Myometrium

 

(n=5 with duplicates)

LPS

0.167*

0.196

0.192*

0.060

PHA

0.149*

0.160*

0.180

0.056

  1. Genes with M values <1.5 are considered stable. Asterisks show the pair of genes from which pairwise variation was calculated. V2/3 <0.15 indicates that those two genes are sufficient for normalization.
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BMC Research Notes

ISSN: 1756-0500

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