Figure 1From: Validation of reference genes for normalization of qPCR gene expression data from Coffea spp. hypocotyls inoculated with Colletotrichum kahawaePairwise variation (V) of candidate genes as predicted by GeNorm. The pairwise variation (Vn/Vn + 1) was calculated between the normalization factors NFn and NFn + 1. Each pairwise variation value is compared with a recommended cut-off value 0.15, below which the inclusion of an additional reference gene is not required. RCRI – Catimor 88 inoculated compared to control samples, SCSI – Caturra inoculated compared to control samples.Back to article page