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Table 6 Alignment statistics of reads mapped against the Variola virus genome

From: False positives complicate ancient pathogen identifications using high-throughput shotgun sequencing

Platform

Sample Description

Reads Mapped (% Mapped)

Molecular Length (bp)

Total Mapped (bp)

HeliScope

Grand Plaza Bulk 1

19 (0.00%)

24.2 ± 0.5

459

 

Grand Plaza Bulk 2

28 (0.00%)

24.7 ± 0.9

692

 

Grand Plaza Phos. 1

11 (0.00%)

24.3 ± 0.5

267

 

Grand Plaza Phos. 2

14 (0.00%)

24.6 ± 1.2

344

 

Churchyard Bulk 1

35 (0.00%)

24.9 ± 2.0

870

 

Churchyard Bulk 2

30 (0.00%)

25.7 ± 5.4

772

 

Churchyard Phos. 1

21 (0.00%)

25.5 ± 3.9

535

 

Churchyard Phos. 2

28 (0.00%)

24.8 ± 2.1

693

 

Grand Plaza Sheared

0 (0.00%)

0

 

Churchyard Sheared

0 (0.00%)

0

 

Soil Bulk

1 (0.00%)

24.0

24

 

Soil Sheared

0 (0.00%)

0

HiSeq 2500

TP04

0 (0.00%)

0

 

TP09

0 (0.00%)

0

 

TP10

0 (0.00%)

0

 

TP18

0 (0.00%)

0

 

TP37

0 (0.00%)

0

 

TP48

0 (0.00%)

0

 

Soil Bulk

0 (0.00%)

0

  1. “Bulk” samples are the unsheared bulks. “Phos.” samples are the phosphatase-treated unsheared bulks. “Sheared” samples are the sheared bulks. “% Mapped” indicates the percentage of the total number of reads comprised by the reads mapped onto the Variola genome. “Molecular Length” is the mean length (± standard deviation) of the mapped reads.