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Table 1 Characteristics for 15 polymorphic microsatellites in the African Buff-spotted Woodpecker Campethera nivosa

From: Isolation and development of microsatellite loci in an African Woodpecker (Campethera nivosa) using polymerase chain reaction and DNA sequencing

Locus

Primer sequence (5′–3′)

Primer labeling

Repeat motif

Ta (°C)

n

HWE

k 

Ho

He

PID

GenBank accession no.

CNI 1*

F: TGTAAAACGACGGCCAGTGGTGGTGGAGTCACCTTCAT

VIC

(GTATT)7

60

15

1

3

0.917

0.531

3.2E−01

KP418965

 

R: GTGTCTTCCTTTACTTGCCCTTCTTGC

          

CNI 2*

F: TGTAAAACGACGGCCAGTCCCCTCTTGGAAGTGTTCAA

VIC

(AAAC)5

60

15

<0.0001

5

0.1

0.485

2.9E−01

KP636531

 

R: GTGTCTTGGGGGAGTTTGACTCAAGTG

          

CNI 3*

F: TGTAAAACGACGGCCAGTAAAGACATCCATTGCCCTTG

6-FAM

(AGACT)12

59

15

<0.0001

7

0.25

0.747

9.6E−02

KP636532

 

R: GTGTCTTTCTTCCAACCTGGTCTGGTC

          

CNI 4

F: TGTAAAACGACGGCCAGTAGACTGGATGGGACACTTGG

VIC

(ATTTCT)11

50

15

0.7509

8

0.818

0.752

9.2E−02

KP418966

 

R: GTGTCTTAGTGGCACCTCCTGAGACAT

          

CNI 6

F: TGTAAAACGACGGCCAGTGCAAAAGGTGGTATTGGAAGA

VIC

(AC)6

60

15

0.3507

4

0.667

0.705

1.4E−01

KP418967

 

R: GTGTCTTTGTGTGCTGGAATAGGCAAG

          

CNI 7

F: TGTAAAACGACGGCCAGTATTTTCCCCCGTCTCTGATT

6-FAM

(TG)6

54

15

0.0072

4

0.273

0.624

1.9E−01

KP418968

 

R: GTGTCTTCAAACGAACATCACCACCAC

          

CNI 8*

F: TGTAAAACGACGGCCAGTTGGATGATAGGTTGGACGTG

VIC

(CTATT)10

59

15

<0.0001

8

0.444

0.852

3.9E−02

KP636533

 

R: GTGTCTTGCCCATCAACAGAAAGCAGT

          

CNI 9

F: TGTAAAACGACGGCCAGTCCTCCTCTAACACCACACCA

VIC

(CA)10

59

15

0.0188

12

0.75

0.889

2.2E−02

KP418969

 

R: GTGTCTTGACCAGGCCAGTGGATTTTA

          

CNI 11

F: TGTAAAACGACGGCCAGTTGGCTCCACACTGAGTTGTC

VIC

(AATAG)12

60

15

0.4846

10

0.917

0.847

4.0E−02

KP418970

 

R: GTGTCTTCGAAGGTCTCTTCCAACCTG

          

CNI 12

F: TGTAAAACGACGGCCAGTACAGCCTCTCCCATTGTCTC

NED

(AGAAT)5

50

15

0.0482

3

0.455

0.632

2.1E−01

KP418971

 

R: GTGTCTTGCTTGGTGCCATGGTTTAGT

          

CNI 13

F: TGTAAAACGACGGCCAGTTTCCAACCTGGTCAATTCAA

VIC

(CTATT)11, (CTACT)10

57

15

0.005

13

0.636

0.901

1.8E−02

KP418972

 

R: GTGTCTTGGCATGCCTAGCTTTGGATA

          

CNI 15

F: TGTAAAACGACGGCCAGTTCTTCCTAGGGCCTGTCACT

NED

(TCTA)7

59

15

0.0034

10

0.667

0.84

4.4E−02

KP418973

 

R: GTGTCTTTCCACTTGAAAGGAAAGAGGTC

          

CNI 16

F: TGTAAAACGACGGCCAGTTTTGACCAAGGAGGGAAAAA

NED

(GATA)8

59

15

0.9271

7

0.75

0.698

1.2E−01

KP418974

 

R: GTGTCTTCAGGGGATTATAGGGGATGG

          

CNI 17

F: TGTAAAACGACGGCCAGTTGGAAGACTGGGACCAAAAC

NED

(ATCT)12

59

15

0.398

10

1

0.865

3.3E−02

KP418975

 

R: GTGTCTTGAATAATCACAATTGTTAATCTGCAT

          

CNI 18

F: TGTAAAACGACGGCCAGTTGGAAGACTGGGACCAAAAC

VIC

(CTAT)12

59

15

0.7442

8

0.833

0.785

7.1E−02

KP418976

 

R: GTGTCTTGAATAATCACAATTGTTAATCTGCAT

          
  1. Ta optimized annealing temperature, n number of individuals genotyped, k number of alleles, Ho observed heterozygosity, He expected heterozygosity, HWE p values from heterozygote deficit tests, PID probability of identity.
  2. * Departure from Hardy–Weinberg equilibrium (in terms of heterozygote deficit) following Bonferroni correction. CNI1 exhibited heterozygote excess (p = 0.01).
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BMC Research Notes

ISSN: 1756-0500

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