Fig. 5

VT treatment decreases the expression of Eotaxin and other important inflammatory cytokines and decreases the number of eosinophils produced in vitro and in vivo. a Plasma from the animals treated as describe previously analyzed by either cytokine bead array for TNF-α, IL-9, IL-13 or MCP-1 or by ELISA for Eotaxin. p < 0.05 was considered statistically significant. Statistics were performed as pair-wise analysis with groups shown significant differences indicated. b Peripheral blood was drawn from animals treated with vehicle or VT for 30 days. Blood was processed for FACS analysis for surface IgE and FITC absorption. The decreased number of basophils and eosinophils in treated normal and AT-Derm mice is clearly evident in noted gates, while no effect was seen on neutrophils. c Dissociated bone marrow from CD1 mice was taken and plated in Methocult GF M3434 media for 7 days. Hematopoietic colonies were enumerated. A dose dependent, statistically significant reduction in CFU-G numbers was noted in response to VT treatment