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Table 2 A collection of forty three successfully amplified primers

From: Variation in sequences containing microsatellite motifs in the perennial biomass and forage grass, Phalaris arundinacea (Poaceae)

Primer name

Isotiq position

Repeat motif

Primer sequence (5′-3′)

Ta

TeaPh_nSSR_1

00060-1

(AC)4

F: TACTTCATTGGGTGGGATGG R: CGCGAATGAAATGAGAAAGC

54

TeaPh_nSSR_2

00060-2

(AT)4

F: GGTGGCTAATCTCAGGAATGG R: TGCCCGATAATAAGCACTAGC

54

TeaPh_nSSR_3

08186-1

(TTG)4

F: GGTAAATTCAGATTATTCCAAAACC R: CCTTTTTGAATGGCAGTTCC

53

TeaPh_nSSR_4

01314-2

(TGT)4

F: AACGGTGACAAAAGACAAAGC R: CAGCCGTATATCCACAATGC

54

TeaPh_nSSR_5

01313-2

(TGT)4

F: AACGGTGACAAAAGACAAAGC R: CAGCCGTATATCCACAATGC

54

TeaPh_nSSR_6

08185-3

(ATT)4

F: TGGCCAACTCTCAGTAGAAGG R: CCATGACCAAAATGAACTCC

53

TeaPh_nSSR_7

00075-2

(TTCT)4

F: TCCCCTCTTTGTTTATCATTCG R: GAATCCGGTAAGGTACTTTTGG

54

TeaPh_nSSR_8

00074-2

(TTCT)4

F: TCCCCTCTTTGTTTATCATTCG R: GAATCCGGTAAGGTACTTTTGG

54

TeaPh_nSSR_9

00068-2

(TTCT)4

F: TCCCCTCTTTGTTTATCATTCG R: GAATCCGGTAAGGTACTTTTGG

54

TeaPh_nSSR_10

03441-1

(TA)4

F: TGCAATGATTTTCTCTATCTTGC R: TCTATCGCTTCACTTTGTCTCG

53

TeaPh_nSSR_11

03440-1

(TA)4

F: TGCAATGATTTTCTCTATCTTGC R: TCTATCGCTTCACTTTGTCTCG

53

TeaPh_nSSR_13

00265-3

(GA)4

F: AGCAAGTATGCCGAAAGACC R: GGGAGACCCACACTTACAGC

54

TeaPh_nSSR_16

03439-1

(CT)4

F: GTACCCGAAACCGACACAGG R: CCCCCATACATGGTCTTACG

55

TeaPh_nSSR_17

03438-1

(CT)4

F: TTCTCCACGAGGCTCATACC R: GAAGTTACGGGGCTATTTTGC

55

TeaPh_nSSR_19

03440-4

(AT)4

F: TTCCGAATTAAATGGAGAATCC R: GATAACGGGACATGAAGACTCC

54

TeaPh_nSSR_20

00072-1

(AT)4

F: GGTGGCTAATCTCAGGAATGG R: TGCCCGATAATAAGCACTAGC

54

TeaPh_nSSR_21

08188-1

(AG)4

F: CAATGCCAAAGAAACAATGC R: ACCTCAGATCGAAGCATTCC

54

TeaPh_nSSR_23

00071-1

(AC)4

F: TACTTCATTGGGTGGGATGG R: CGCGAATGAAATGAGAAAGC

56

TeaPh_nSSR_24

03440-3

(AT)5

F: GAATGAAAATGCCAATAAAGTCG R: TTTTATTTCTCTAATTCGCAAATCC

54

TeaPh_nSSR_25

08351-5

(TGC)10

F: TCCTATGATCTCTGCCTCAGC R: GCACTGTCCATCAACACACC

55

TeaPh_nSSR_34

01672-8

(CCGAAACA)3

F: TTACCGACTCCGTCTTGACC R: GTCGATGGAGATGACGTTGG

55

TeaPh_nSSR_37

03471-10

(TTTTGAA)3

F: GTGTTTGGCCTGTAATCTGG R: CGTAAATGCATCTCTATCTGTTCC

53

TeaPh_nSSR_42

01705-4

(GT)8

F: TCAAGTGTCATCCGTTGTCC R: TTTTAACGCAAATAGTTTCATCG

53

TeaPh_nSSR_43

02516-1

(GT)6

F: TGGACTGCACCTAGGAGACC R: TACCACCATGGAACAAAACG

54

TeaPh_nSSR_45

08327-5

(GC)4

F: AAAGTACATTGAAAGCTAGTGTCACC R: GCCTCCAAAGCAAGATGC

54

TeaPh_nSSR_46

03588-3

(CG)6

F: TCTCCGCTCGATCTAAATAGC R: TGTGTGTGCTGAAAGTGTCG

55

TeaPh_nSSR_47

01700-4

(CG)4

F: GACAGATGGGGCACTACTCC R: GTGTGAGGAATCCACAGTGC

54

TeaPh_nSSR_48

02594-3

(TAA)6

F: AAGAGTGTCACCATGGAGTGG R: ACCTTCTGAGAGCCTCTTGC

54

TeaPh_nSSR_49

02597-9

(GCA)5

F: GATACGCTGGAATACCAGAAGG R: GGGAATGGAAACGAACAGG

55

TeaPh_nSSR_50

08302-4

(GAA)5

F: AAGAGGAAGCCGAAGAGTGG R: TCTGTGGTGCTCAGTTCAGG

55

TeaPh_nSSR_52

08189-10

(GAT)3

F: TTAACTCGAGGTCATGCATCC R: CCTTTAGCGTCCAAAACTGC

55

TeaPh_nSSR_54

08427-4

(CAA)4

F: ACATCCACAGGATTCCATGC R: GCCAGAGATGAGAAGGATGC

55

TeaPh_nSSR_55

02553-8

(CAT)3

F: AGCAACCAGAACCTGACACG R: AGATGGTACGGCTGGTATGC

55

TeaPh_nSSR_57

02609-7

(CGG)4

F: GTTCGCTTCGATTTGTTTCC R: CGAAATGAACGGCCTAATCC

55

TeaPh_nSSR_58

08459-13

(GCTC)4

F: TCCCGACTTCATGAGCTACC R: GGAGGAGCATGTGTGAATGG

55

TeaPh_nSSR_59

00075-2

(TTCT)4

F: TCCCCTCTTTGTTTATCATTCG R: GAATCCGGTAAGGTACTTTTGG

54

TeaPh_nSSR_60

01318-11

(AGGA)3

F: GGGCTTTCTACATAGGGATCG R: TTGATCTTTACGGTGCTTTCC

54

TeaPh_nSSR_65

08352-6

(AGG)4

F: CTCCACCACCTCCACAAAAT R: TTTCGTCTTTGTGCTTGCTG

55

TeaPh_nSSR_66

00769-7

(TTG)3

F: CGTTGTGCCTTAGCTACTTGC R: ATGATCCAACCAGCTTGACC

55

TeaPh_nSSR_70

08235-11

(TGCT)3

F: CCTTGAGGAGGATGATGTGG R: TCCTGATGTGCTTGATGAGC

55

TeaPh_nSSR_71

08212-11

(TTCA)3

F: GATGGAATCACGCTCTGTAGG R: GGGCAGTAGCGAAGAGATCC

55

TeaPh_nSSR_80

03674-1

(GAT)4

F: CCAAACCCAGTTGTGACTCC R: GGCATCAGAATCATAGTCATCG

55

TeaPh_nSSR_81

03659-2

(GGT)5

F: CGGTTGGACTGATAACATTGG R: CCCATCCTGAGTCGTCACC

55

  1. Forty three primers that successfully amplified a distinct band of expected size on a 3 % MetaPhor agarose gel, their Isotiq position on the EST assembly, repeat motif, forward and reverse primer sequences (5′-3′) and annealing temperature (Ta)